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STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEM : III. DEVELOPMENT OF INFECTIVITY, HEMAGGLUTINATION, AND COMPLEMENT FIXATION ACTIVITIES DURING THE FIRST INFECTIOUS CYCLE
In agreement with earlier observations the infectivity titer in the allantoic fluids of chick embryos injected with influenza A virus remains constant for 5 to 6 hours before an increase in this activity can be noted. In contrast, the titers of hemagglutinin and complement-fixing antigen (virus anti...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1949
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135936/ https://www.ncbi.nlm.nih.gov/pubmed/18152337 |
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author | Henle, Werner Henle, Gertrude |
author_facet | Henle, Werner Henle, Gertrude |
author_sort | Henle, Werner |
collection | PubMed |
description | In agreement with earlier observations the infectivity titer in the allantoic fluids of chick embryos injected with influenza A virus remains constant for 5 to 6 hours before an increase in this activity can be noted. In contrast, the titers of hemagglutinin and complement-fixing antigen (virus antigen) have already begun to rise after 3 hours. The origin of the hemagglutinating and complement-fixing but non-infectious material is still obscure. In the allantoic membrane development of both the soluble and virus antigens can be demonstrated after the 2nd hour of incubation and 1 hour prior to an increase in hemagglutinins and 2 hours prior to a rise in infectivity. Thus there remain the first 2 hours during which no record of virus activity in the tissues can be obtained. Similar relationships are noted on dilution of the seed both in the allantoic fluids and membranes as long as the various properties reach measurable levels during the experimental period of one infectious cycle. Injection of high titered immune serum following infection with about 10(9) ID(50) reduces the amount of demonstrable seed virus in the allantoic fluid and membrane without significantly affecting propagation of the agent in the tissues as measured by infectivity titrations. The production of hemagglutinins appears markedly reduced under these conditions whereas formation of complement-fixing antigen is only slightly delayed and decreased. No definite explanations for the various discrepancies between the infectivity and hemagglutination can be given at present. |
format | Text |
id | pubmed-2135936 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1949 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21359362008-04-17 STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEM : III. DEVELOPMENT OF INFECTIVITY, HEMAGGLUTINATION, AND COMPLEMENT FIXATION ACTIVITIES DURING THE FIRST INFECTIOUS CYCLE Henle, Werner Henle, Gertrude J Exp Med Article In agreement with earlier observations the infectivity titer in the allantoic fluids of chick embryos injected with influenza A virus remains constant for 5 to 6 hours before an increase in this activity can be noted. In contrast, the titers of hemagglutinin and complement-fixing antigen (virus antigen) have already begun to rise after 3 hours. The origin of the hemagglutinating and complement-fixing but non-infectious material is still obscure. In the allantoic membrane development of both the soluble and virus antigens can be demonstrated after the 2nd hour of incubation and 1 hour prior to an increase in hemagglutinins and 2 hours prior to a rise in infectivity. Thus there remain the first 2 hours during which no record of virus activity in the tissues can be obtained. Similar relationships are noted on dilution of the seed both in the allantoic fluids and membranes as long as the various properties reach measurable levels during the experimental period of one infectious cycle. Injection of high titered immune serum following infection with about 10(9) ID(50) reduces the amount of demonstrable seed virus in the allantoic fluid and membrane without significantly affecting propagation of the agent in the tissues as measured by infectivity titrations. The production of hemagglutinins appears markedly reduced under these conditions whereas formation of complement-fixing antigen is only slightly delayed and decreased. No definite explanations for the various discrepancies between the infectivity and hemagglutination can be given at present. The Rockefeller University Press 1949-06-30 /pmc/articles/PMC2135936/ /pubmed/18152337 Text en Copyright © Copyright, 1949, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Henle, Werner Henle, Gertrude STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEM : III. DEVELOPMENT OF INFECTIVITY, HEMAGGLUTINATION, AND COMPLEMENT FIXATION ACTIVITIES DURING THE FIRST INFECTIOUS CYCLE |
title | STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEM : III. DEVELOPMENT OF INFECTIVITY, HEMAGGLUTINATION, AND COMPLEMENT FIXATION ACTIVITIES DURING THE FIRST INFECTIOUS CYCLE |
title_full | STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEM : III. DEVELOPMENT OF INFECTIVITY, HEMAGGLUTINATION, AND COMPLEMENT FIXATION ACTIVITIES DURING THE FIRST INFECTIOUS CYCLE |
title_fullStr | STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEM : III. DEVELOPMENT OF INFECTIVITY, HEMAGGLUTINATION, AND COMPLEMENT FIXATION ACTIVITIES DURING THE FIRST INFECTIOUS CYCLE |
title_full_unstemmed | STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEM : III. DEVELOPMENT OF INFECTIVITY, HEMAGGLUTINATION, AND COMPLEMENT FIXATION ACTIVITIES DURING THE FIRST INFECTIOUS CYCLE |
title_short | STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEM : III. DEVELOPMENT OF INFECTIVITY, HEMAGGLUTINATION, AND COMPLEMENT FIXATION ACTIVITIES DURING THE FIRST INFECTIOUS CYCLE |
title_sort | studies on host-virus interactions in the chick embryo-influenza virus system : iii. development of infectivity, hemagglutination, and complement fixation activities during the first infectious cycle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135936/ https://www.ncbi.nlm.nih.gov/pubmed/18152337 |
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