LOCALIZATION OF ANTIGEN IN TISSUE CELLS : II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY

Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two...

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Detalles Bibliográficos
Autores principales: Coons, Albert H., Kaplan, Melvin H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1950
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135948/
https://www.ncbi.nlm.nih.gov/pubmed/15395569
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author Coons, Albert H.
Kaplan, Melvin H.
author_facet Coons, Albert H.
Kaplan, Melvin H.
author_sort Coons, Albert H.
collection PubMed
description Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.
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spelling pubmed-21359482008-04-17 LOCALIZATION OF ANTIGEN IN TISSUE CELLS : II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY Coons, Albert H. Kaplan, Melvin H. J Exp Med Article Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described. The Rockefeller University Press 1950-01-01 /pmc/articles/PMC2135948/ /pubmed/15395569 Text en Copyright © Copyright, 1950, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Coons, Albert H.
Kaplan, Melvin H.
LOCALIZATION OF ANTIGEN IN TISSUE CELLS : II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY
title LOCALIZATION OF ANTIGEN IN TISSUE CELLS : II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY
title_full LOCALIZATION OF ANTIGEN IN TISSUE CELLS : II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY
title_fullStr LOCALIZATION OF ANTIGEN IN TISSUE CELLS : II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY
title_full_unstemmed LOCALIZATION OF ANTIGEN IN TISSUE CELLS : II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY
title_short LOCALIZATION OF ANTIGEN IN TISSUE CELLS : II. IMPROVEMENTS IN A METHOD FOR THE DETECTION OF ANTIGEN BY MEANS OF FLUORESCENT ANTIBODY
title_sort localization of antigen in tissue cells : ii. improvements in a method for the detection of antigen by means of fluorescent antibody
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135948/
https://www.ncbi.nlm.nih.gov/pubmed/15395569
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AT kaplanmelvinh localizationofantigenintissuecellsiiimprovementsinamethodforthedetectionofantigenbymeansoffluorescentantibody

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