PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : I. PRODUCTION AND VIABILITY OF THE CULTURE

Diffuse, submerged growth of BCG bacilli has been obtained in liquid media containing 0.02 per cent Tween 80 and the soluble fraction of human serum heated under acid conditions (pH 2.5) at 65°C. In the absence of glucose or glycerine,—which had a detrimental effect on viability—these cultures consi...

Descripción completa

Detalles Bibliográficos
Autores principales: Dubos, René J., Fenner, Frank
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1950
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135966/
https://www.ncbi.nlm.nih.gov/pubmed/19871703
_version_ 1782143018610982912
author Dubos, René J.
Fenner, Frank
author_facet Dubos, René J.
Fenner, Frank
author_sort Dubos, René J.
collection PubMed
description Diffuse, submerged growth of BCG bacilli has been obtained in liquid media containing 0.02 per cent Tween 80 and the soluble fraction of human serum heated under acid conditions (pH 2.5) at 65°C. In the absence of glucose or glycerine,—which had a detrimental effect on viability—these cultures consisted predominantly of cells that were living and that remained viable during prolonged storage at temperatures ranging from 4 to 37°C.
format Text
id pubmed-2135966
institution National Center for Biotechnology Information
language English
publishDate 1950
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21359662008-04-17 PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : I. PRODUCTION AND VIABILITY OF THE CULTURE Dubos, René J. Fenner, Frank J Exp Med Article Diffuse, submerged growth of BCG bacilli has been obtained in liquid media containing 0.02 per cent Tween 80 and the soluble fraction of human serum heated under acid conditions (pH 2.5) at 65°C. In the absence of glucose or glycerine,—which had a detrimental effect on viability—these cultures consisted predominantly of cells that were living and that remained viable during prolonged storage at temperatures ranging from 4 to 37°C. The Rockefeller University Press 1950-02-28 /pmc/articles/PMC2135966/ /pubmed/19871703 Text en Copyright © Copyright, 1950, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Dubos, René J.
Fenner, Frank
PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : I. PRODUCTION AND VIABILITY OF THE CULTURE
title PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : I. PRODUCTION AND VIABILITY OF THE CULTURE
title_full PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : I. PRODUCTION AND VIABILITY OF THE CULTURE
title_fullStr PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : I. PRODUCTION AND VIABILITY OF THE CULTURE
title_full_unstemmed PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : I. PRODUCTION AND VIABILITY OF THE CULTURE
title_short PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : I. PRODUCTION AND VIABILITY OF THE CULTURE
title_sort production of bcg vaccine in a liquid medium containing tween 80 and a soluble fraction of heated human serum : i. production and viability of the culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135966/
https://www.ncbi.nlm.nih.gov/pubmed/19871703
work_keys_str_mv AT dubosrenej productionofbcgvaccineinaliquidmediumcontainingtween80andasolublefractionofheatedhumanserumiproductionandviabilityoftheculture
AT fennerfrank productionofbcgvaccineinaliquidmediumcontainingtween80andasolublefractionofheatedhumanserumiproductionandviabilityoftheculture

Ejemplares similares