CARBOHYDRATE HISTOCHEMISTRY STUDIED BY ACETYLATION TECHNIQUES : I. PERIODIC ACID METHODS

An acetic anhydride pyridine mixture acetylates hydroxyl groups in tissue sections. The acetyl groups can be removed from tissue sections by weak alkali. Sections acetylated by acetic anhydride pyridine no longer produce aldehydes after oxidation by periodic acid. Acetylated sections subjected to we...

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Detalles Bibliográficos
Autores principales: McManus, J. F. A., Cason, Jane E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1950
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136018/
https://www.ncbi.nlm.nih.gov/pubmed/15422090
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author McManus, J. F. A.
Cason, Jane E.
author_facet McManus, J. F. A.
Cason, Jane E.
author_sort McManus, J. F. A.
collection PubMed
description An acetic anhydride pyridine mixture acetylates hydroxyl groups in tissue sections. The acetyl groups can be removed from tissue sections by weak alkali. Sections acetylated by acetic anhydride pyridine no longer produce aldehydes after oxidation by periodic acid. Acetylated sections subjected to weak alkali regain the ability to produce aldehydes after oxidation by periodic acid. Acetylation of tissue sections and the removal of acetyl groups by weak alkali can be used as histochemical confirmation of the carbohydrate nature of materials coloring with Schiff's reagent after periodic acid.
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spelling pubmed-21360182008-04-17 CARBOHYDRATE HISTOCHEMISTRY STUDIED BY ACETYLATION TECHNIQUES : I. PERIODIC ACID METHODS McManus, J. F. A. Cason, Jane E. J Exp Med Article An acetic anhydride pyridine mixture acetylates hydroxyl groups in tissue sections. The acetyl groups can be removed from tissue sections by weak alkali. Sections acetylated by acetic anhydride pyridine no longer produce aldehydes after oxidation by periodic acid. Acetylated sections subjected to weak alkali regain the ability to produce aldehydes after oxidation by periodic acid. Acetylation of tissue sections and the removal of acetyl groups by weak alkali can be used as histochemical confirmation of the carbohydrate nature of materials coloring with Schiff's reagent after periodic acid. The Rockefeller University Press 1950-06-01 /pmc/articles/PMC2136018/ /pubmed/15422090 Text en Copyright © Copyright, 1950, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
McManus, J. F. A.
Cason, Jane E.
CARBOHYDRATE HISTOCHEMISTRY STUDIED BY ACETYLATION TECHNIQUES : I. PERIODIC ACID METHODS
title CARBOHYDRATE HISTOCHEMISTRY STUDIED BY ACETYLATION TECHNIQUES : I. PERIODIC ACID METHODS
title_full CARBOHYDRATE HISTOCHEMISTRY STUDIED BY ACETYLATION TECHNIQUES : I. PERIODIC ACID METHODS
title_fullStr CARBOHYDRATE HISTOCHEMISTRY STUDIED BY ACETYLATION TECHNIQUES : I. PERIODIC ACID METHODS
title_full_unstemmed CARBOHYDRATE HISTOCHEMISTRY STUDIED BY ACETYLATION TECHNIQUES : I. PERIODIC ACID METHODS
title_short CARBOHYDRATE HISTOCHEMISTRY STUDIED BY ACETYLATION TECHNIQUES : I. PERIODIC ACID METHODS
title_sort carbohydrate histochemistry studied by acetylation techniques : i. periodic acid methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136018/
https://www.ncbi.nlm.nih.gov/pubmed/15422090
work_keys_str_mv AT mcmanusjfa carbohydratehistochemistrystudiedbyacetylationtechniquesiperiodicacidmethods
AT casonjanee carbohydratehistochemistrystudiedbyacetylationtechniquesiperiodicacidmethods

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