STUDIES ON PEROXIDATIVE DETOXIFICATION OF PURIFIED DIPHTHERIA TOXIN

The mechanism of the peroxidative oxidation of diphtheria toxin has been investigated. It has been found that this reaction requires the presence of an oxidizable, dialyzable cofactor in addition to hydrogen peroxide and peroxidase. Cofactors are present in a variety of materials and have been parti...

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Autor principal: Agner, Kjell
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1950
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136044/
https://www.ncbi.nlm.nih.gov/pubmed/14778915
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author Agner, Kjell
author_facet Agner, Kjell
author_sort Agner, Kjell
collection PubMed
description The mechanism of the peroxidative oxidation of diphtheria toxin has been investigated. It has been found that this reaction requires the presence of an oxidizable, dialyzable cofactor in addition to hydrogen peroxide and peroxidase. Cofactors are present in a variety of materials and have been partially purified from urine. The flocculation titer of toxin modified by peroxidation remains unchanged even after 99 per cent or more of the toxicity has been destroyed. The change thus appears analogous to the conversion of toxin to toxoid in the presence of formalin. It is suggested that the well known conversion of crude diphtheria toxin to toxoid which occurs on storage or aging may be due to a slow peroxidative process.
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spelling pubmed-21360442008-04-17 STUDIES ON PEROXIDATIVE DETOXIFICATION OF PURIFIED DIPHTHERIA TOXIN Agner, Kjell J Exp Med Article The mechanism of the peroxidative oxidation of diphtheria toxin has been investigated. It has been found that this reaction requires the presence of an oxidizable, dialyzable cofactor in addition to hydrogen peroxide and peroxidase. Cofactors are present in a variety of materials and have been partially purified from urine. The flocculation titer of toxin modified by peroxidation remains unchanged even after 99 per cent or more of the toxicity has been destroyed. The change thus appears analogous to the conversion of toxin to toxoid in the presence of formalin. It is suggested that the well known conversion of crude diphtheria toxin to toxoid which occurs on storage or aging may be due to a slow peroxidative process. The Rockefeller University Press 1950-09-30 /pmc/articles/PMC2136044/ /pubmed/14778915 Text en Copyright © Copyright, 1950, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Agner, Kjell
STUDIES ON PEROXIDATIVE DETOXIFICATION OF PURIFIED DIPHTHERIA TOXIN
title STUDIES ON PEROXIDATIVE DETOXIFICATION OF PURIFIED DIPHTHERIA TOXIN
title_full STUDIES ON PEROXIDATIVE DETOXIFICATION OF PURIFIED DIPHTHERIA TOXIN
title_fullStr STUDIES ON PEROXIDATIVE DETOXIFICATION OF PURIFIED DIPHTHERIA TOXIN
title_full_unstemmed STUDIES ON PEROXIDATIVE DETOXIFICATION OF PURIFIED DIPHTHERIA TOXIN
title_short STUDIES ON PEROXIDATIVE DETOXIFICATION OF PURIFIED DIPHTHERIA TOXIN
title_sort studies on peroxidative detoxification of purified diphtheria toxin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136044/
https://www.ncbi.nlm.nih.gov/pubmed/14778915
work_keys_str_mv AT agnerkjell studiesonperoxidativedetoxificationofpurifieddiphtheriatoxin

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