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DETERMINATION OF INHERITED TRAITS OF H. INFLUENZAE BY DESOXYRIBONUCLEIC ACID FRACTIONS ISOLATED FROM TYPE-SPECIFIC CELLS
Change of non-typable (R) strains of H. influenzae into the specific type of origin or new types (S) has been effected in vitro in a predictable fashion within a single 24 hour growth period, by a desoxyribonucleic acid-containing fraction isolated from type-specific cells of the type desired. Only...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1951
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136084/ https://www.ncbi.nlm.nih.gov/pubmed/14824407 |
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author | Alexander, Hattie E. Leidy, Grace |
author_facet | Alexander, Hattie E. Leidy, Grace |
author_sort | Alexander, Hattie E. |
collection | PubMed |
description | Change of non-typable (R) strains of H. influenzae into the specific type of origin or new types (S) has been effected in vitro in a predictable fashion within a single 24 hour growth period, by a desoxyribonucleic acid-containing fraction isolated from type-specific cells of the type desired. Only a small proportion of the population of R cells are susceptible to the change induced in inherited characteristics by the desoxyribonucleic acid fraction. The data suggest that the number of susceptible cells present in any given population size varies with the specific type of origin of the R cells; a lesser degree of variation in different independent cultures of the same strain and population size has been demonstrated. The results suggest that the R H. influenzae cells which are susceptible to transformation arise as the result of mutation. It has been demonstrated that the reaction necessary for transformation takes place virtually immediately if susceptible cells are present. Furthermore it has been shown that the change which enables an R cell to form a colony of type-specific organisms has been completed within 15 minutes in an environment which does not permit cell multiplication. |
format | Text |
id | pubmed-2136084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1951 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21360842008-04-17 DETERMINATION OF INHERITED TRAITS OF H. INFLUENZAE BY DESOXYRIBONUCLEIC ACID FRACTIONS ISOLATED FROM TYPE-SPECIFIC CELLS Alexander, Hattie E. Leidy, Grace J Exp Med Article Change of non-typable (R) strains of H. influenzae into the specific type of origin or new types (S) has been effected in vitro in a predictable fashion within a single 24 hour growth period, by a desoxyribonucleic acid-containing fraction isolated from type-specific cells of the type desired. Only a small proportion of the population of R cells are susceptible to the change induced in inherited characteristics by the desoxyribonucleic acid fraction. The data suggest that the number of susceptible cells present in any given population size varies with the specific type of origin of the R cells; a lesser degree of variation in different independent cultures of the same strain and population size has been demonstrated. The results suggest that the R H. influenzae cells which are susceptible to transformation arise as the result of mutation. It has been demonstrated that the reaction necessary for transformation takes place virtually immediately if susceptible cells are present. Furthermore it has been shown that the change which enables an R cell to form a colony of type-specific organisms has been completed within 15 minutes in an environment which does not permit cell multiplication. The Rockefeller University Press 1951-03-31 /pmc/articles/PMC2136084/ /pubmed/14824407 Text en Copyright © Copyright, 1951, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Alexander, Hattie E. Leidy, Grace DETERMINATION OF INHERITED TRAITS OF H. INFLUENZAE BY DESOXYRIBONUCLEIC ACID FRACTIONS ISOLATED FROM TYPE-SPECIFIC CELLS |
title | DETERMINATION OF INHERITED TRAITS OF H. INFLUENZAE BY DESOXYRIBONUCLEIC ACID FRACTIONS ISOLATED FROM TYPE-SPECIFIC CELLS |
title_full | DETERMINATION OF INHERITED TRAITS OF H. INFLUENZAE BY DESOXYRIBONUCLEIC ACID FRACTIONS ISOLATED FROM TYPE-SPECIFIC CELLS |
title_fullStr | DETERMINATION OF INHERITED TRAITS OF H. INFLUENZAE BY DESOXYRIBONUCLEIC ACID FRACTIONS ISOLATED FROM TYPE-SPECIFIC CELLS |
title_full_unstemmed | DETERMINATION OF INHERITED TRAITS OF H. INFLUENZAE BY DESOXYRIBONUCLEIC ACID FRACTIONS ISOLATED FROM TYPE-SPECIFIC CELLS |
title_short | DETERMINATION OF INHERITED TRAITS OF H. INFLUENZAE BY DESOXYRIBONUCLEIC ACID FRACTIONS ISOLATED FROM TYPE-SPECIFIC CELLS |
title_sort | determination of inherited traits of h. influenzae by desoxyribonucleic acid fractions isolated from type-specific cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136084/ https://www.ncbi.nlm.nih.gov/pubmed/14824407 |
work_keys_str_mv | AT alexanderhattiee determinationofinheritedtraitsofhinfluenzaebydesoxyribonucleicacidfractionsisolatedfromtypespecificcells AT leidygrace determinationofinheritedtraitsofhinfluenzaebydesoxyribonucleicacidfractionsisolatedfromtypespecificcells |