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THE INTRACELLULAR LOCALIZATION OF POLIOMYELITIS VIRUS

A study was made of the intracellular localization of Type 2 poliomyelitis virus, using the technique of Mirsky and Pollister (23) for cellular fractionation. After isotonic saline homogenization of central nervous system tissue from infected mice, and subsequent centrifugation of the suspension, th...

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Autores principales: Kaplan, Albert S., Melnick, Joseph L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1953
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136186/
https://www.ncbi.nlm.nih.gov/pubmed/13022865
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author Kaplan, Albert S.
Melnick, Joseph L.
author_facet Kaplan, Albert S.
Melnick, Joseph L.
author_sort Kaplan, Albert S.
collection PubMed
description A study was made of the intracellular localization of Type 2 poliomyelitis virus, using the technique of Mirsky and Pollister (23) for cellular fractionation. After isotonic saline homogenization of central nervous system tissue from infected mice, and subsequent centrifugation of the suspension, the virus present in the supernatant fluid was held to be of cytoplasmic origin. Upon serial washings of the sediment with physiological saline, the resulting supernates contained progressively less virus until by the seventh washing, virtually none was present. At this point extraction of the washed sediment with molar NaCl, which lyses the nuclei, yielded substantial amounts of virus, and this was assumed to be from nuclear sources. The possibility has not been excluded however that the "nuclear" sediment was contaminated by cytoplasmic particles too large to remain in the supernate. Experiments on the increase of virus during the incubation and acute stages of infection have revealed that it was first detectable in the "cytoplasmic" fraction and subsequently in the "nuclear" fraction. Virus in the "nuclear" fraction from paralyzed mice sometimes reached titers almost as high as those found in the "cytoplasm." Adsorption experiments indicated that the "nuclear" fraction of CNS tissue from normal, uninoculated mice did not adsorb added Type 2 poliomyelitis virus, nor did such fractions adsorb virus procured from the "cytoplasm" or "nuclei" of infected cells. Although individual mice varied in their response after virus injection, the "cytoplasmic" fraction of paralytic mice was found to contain virus regularly, whereas little more than half of the non-paralytic mice yielded it. When virus was present in the "cytoplasm," it could be found in the "nuclear" fraction of paralytic mice with much greater regularity than in that of non-paralytic mice. A comparison between the lines of the MEF1 strain of poliomyelitis virus, "adapted" and "non-adapted" to newborn mice, and the Lansing strain, revealed no differences in their intracellular increase. In both infant and adult mice, the chief difference in the findings with non-paralyzed and paralyzed mice lay in the greater concentration of virus in the "nuclear" fractions of the latter group.
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spelling pubmed-21361862008-04-17 THE INTRACELLULAR LOCALIZATION OF POLIOMYELITIS VIRUS Kaplan, Albert S. Melnick, Joseph L. J Exp Med Article A study was made of the intracellular localization of Type 2 poliomyelitis virus, using the technique of Mirsky and Pollister (23) for cellular fractionation. After isotonic saline homogenization of central nervous system tissue from infected mice, and subsequent centrifugation of the suspension, the virus present in the supernatant fluid was held to be of cytoplasmic origin. Upon serial washings of the sediment with physiological saline, the resulting supernates contained progressively less virus until by the seventh washing, virtually none was present. At this point extraction of the washed sediment with molar NaCl, which lyses the nuclei, yielded substantial amounts of virus, and this was assumed to be from nuclear sources. The possibility has not been excluded however that the "nuclear" sediment was contaminated by cytoplasmic particles too large to remain in the supernate. Experiments on the increase of virus during the incubation and acute stages of infection have revealed that it was first detectable in the "cytoplasmic" fraction and subsequently in the "nuclear" fraction. Virus in the "nuclear" fraction from paralyzed mice sometimes reached titers almost as high as those found in the "cytoplasm." Adsorption experiments indicated that the "nuclear" fraction of CNS tissue from normal, uninoculated mice did not adsorb added Type 2 poliomyelitis virus, nor did such fractions adsorb virus procured from the "cytoplasm" or "nuclei" of infected cells. Although individual mice varied in their response after virus injection, the "cytoplasmic" fraction of paralytic mice was found to contain virus regularly, whereas little more than half of the non-paralytic mice yielded it. When virus was present in the "cytoplasm," it could be found in the "nuclear" fraction of paralytic mice with much greater regularity than in that of non-paralytic mice. A comparison between the lines of the MEF1 strain of poliomyelitis virus, "adapted" and "non-adapted" to newborn mice, and the Lansing strain, revealed no differences in their intracellular increase. In both infant and adult mice, the chief difference in the findings with non-paralyzed and paralyzed mice lay in the greater concentration of virus in the "nuclear" fractions of the latter group. The Rockefeller University Press 1953-01-01 /pmc/articles/PMC2136186/ /pubmed/13022865 Text en Copyright © Copyright, 1953, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kaplan, Albert S.
Melnick, Joseph L.
THE INTRACELLULAR LOCALIZATION OF POLIOMYELITIS VIRUS
title THE INTRACELLULAR LOCALIZATION OF POLIOMYELITIS VIRUS
title_full THE INTRACELLULAR LOCALIZATION OF POLIOMYELITIS VIRUS
title_fullStr THE INTRACELLULAR LOCALIZATION OF POLIOMYELITIS VIRUS
title_full_unstemmed THE INTRACELLULAR LOCALIZATION OF POLIOMYELITIS VIRUS
title_short THE INTRACELLULAR LOCALIZATION OF POLIOMYELITIS VIRUS
title_sort intracellular localization of poliomyelitis virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136186/
https://www.ncbi.nlm.nih.gov/pubmed/13022865
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