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MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG
When monocytes derived from normal guinea pigs or rabbits were infected with tubercle bacilli and cultivated in vitro, the bacilli multiplied abundantly within the cytoplasm of these cells. By contrast, intracellular multiplication of the bacilli was retarded or completely inhibited within the monoc...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1953
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136193/ https://www.ncbi.nlm.nih.gov/pubmed/13022876 |
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author | Suter, Emanuel |
author_facet | Suter, Emanuel |
author_sort | Suter, Emanuel |
collection | PubMed |
description | When monocytes derived from normal guinea pigs or rabbits were infected with tubercle bacilli and cultivated in vitro, the bacilli multiplied abundantly within the cytoplasm of these cells. By contrast, intracellular multiplication of the bacilli was retarded or completely inhibited within the monocytes of rabbits or guinea pigs vaccinated with BCG. This inhibition of growth was observed with both virulent or attenuated strains of tubercle bacilli. Under the conditions used in the present study, the ability of monocytes to inhibit bacillary proliferation was the same whether serum from a normal animal or from vaccinated animals was used in the tissue culture medium. Moreover, the serum of vaccinated animals did not inhibit multiplication of tubercle bacilli within monocytes derived from a normal animal. The ability of guinea pig monocytes to interfere with intracellular bacillary proliferation was first perceptible 8 days after vaccination. |
format | Text |
id | pubmed-2136193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1953 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21361932008-04-17 MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG Suter, Emanuel J Exp Med Article When monocytes derived from normal guinea pigs or rabbits were infected with tubercle bacilli and cultivated in vitro, the bacilli multiplied abundantly within the cytoplasm of these cells. By contrast, intracellular multiplication of the bacilli was retarded or completely inhibited within the monocytes of rabbits or guinea pigs vaccinated with BCG. This inhibition of growth was observed with both virulent or attenuated strains of tubercle bacilli. Under the conditions used in the present study, the ability of monocytes to inhibit bacillary proliferation was the same whether serum from a normal animal or from vaccinated animals was used in the tissue culture medium. Moreover, the serum of vaccinated animals did not inhibit multiplication of tubercle bacilli within monocytes derived from a normal animal. The ability of guinea pig monocytes to interfere with intracellular bacillary proliferation was first perceptible 8 days after vaccination. The Rockefeller University Press 1953-01-31 /pmc/articles/PMC2136193/ /pubmed/13022876 Text en Copyright © Copyright, 1953, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Suter, Emanuel MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG |
title | MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG |
title_full | MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG |
title_fullStr | MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG |
title_full_unstemmed | MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG |
title_short | MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG |
title_sort | multiplication of tubercle bacilli within mononuclear phagocytes in tissue cultures derived from normal animals and animals vaccinated with bcg |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136193/ https://www.ncbi.nlm.nih.gov/pubmed/13022876 |
work_keys_str_mv | AT suteremanuel multiplicationoftuberclebacilliwithinmononuclearphagocytesintissueculturesderivedfromnormalanimalsandanimalsvaccinatedwithbcg |