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MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG

When monocytes derived from normal guinea pigs or rabbits were infected with tubercle bacilli and cultivated in vitro, the bacilli multiplied abundantly within the cytoplasm of these cells. By contrast, intracellular multiplication of the bacilli was retarded or completely inhibited within the monoc...

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Detalles Bibliográficos
Autor principal: Suter, Emanuel
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1953
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136193/
https://www.ncbi.nlm.nih.gov/pubmed/13022876
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author Suter, Emanuel
author_facet Suter, Emanuel
author_sort Suter, Emanuel
collection PubMed
description When monocytes derived from normal guinea pigs or rabbits were infected with tubercle bacilli and cultivated in vitro, the bacilli multiplied abundantly within the cytoplasm of these cells. By contrast, intracellular multiplication of the bacilli was retarded or completely inhibited within the monocytes of rabbits or guinea pigs vaccinated with BCG. This inhibition of growth was observed with both virulent or attenuated strains of tubercle bacilli. Under the conditions used in the present study, the ability of monocytes to inhibit bacillary proliferation was the same whether serum from a normal animal or from vaccinated animals was used in the tissue culture medium. Moreover, the serum of vaccinated animals did not inhibit multiplication of tubercle bacilli within monocytes derived from a normal animal. The ability of guinea pig monocytes to interfere with intracellular bacillary proliferation was first perceptible 8 days after vaccination.
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spelling pubmed-21361932008-04-17 MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG Suter, Emanuel J Exp Med Article When monocytes derived from normal guinea pigs or rabbits were infected with tubercle bacilli and cultivated in vitro, the bacilli multiplied abundantly within the cytoplasm of these cells. By contrast, intracellular multiplication of the bacilli was retarded or completely inhibited within the monocytes of rabbits or guinea pigs vaccinated with BCG. This inhibition of growth was observed with both virulent or attenuated strains of tubercle bacilli. Under the conditions used in the present study, the ability of monocytes to inhibit bacillary proliferation was the same whether serum from a normal animal or from vaccinated animals was used in the tissue culture medium. Moreover, the serum of vaccinated animals did not inhibit multiplication of tubercle bacilli within monocytes derived from a normal animal. The ability of guinea pig monocytes to interfere with intracellular bacillary proliferation was first perceptible 8 days after vaccination. The Rockefeller University Press 1953-01-31 /pmc/articles/PMC2136193/ /pubmed/13022876 Text en Copyright © Copyright, 1953, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Suter, Emanuel
MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG
title MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG
title_full MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG
title_fullStr MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG
title_full_unstemmed MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG
title_short MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG
title_sort multiplication of tubercle bacilli within mononuclear phagocytes in tissue cultures derived from normal animals and animals vaccinated with bcg
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136193/
https://www.ncbi.nlm.nih.gov/pubmed/13022876
work_keys_str_mv AT suteremanuel multiplicationoftuberclebacilliwithinmononuclearphagocytesintissueculturesderivedfromnormalanimalsandanimalsvaccinatedwithbcg