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QUANTITATIVE ASPECTS OF THE SPONTANEOUS ELUTION OF INFLUENZA VIRUS FROM RED CELLS
Each chick and human red cell contains approximately 300 sites capable of attaching influenza virus particles. These correspond to an area representing approximately 2 per cent of the red cell surface. Although the rate of attachment of PR8 to red cells is not diffusion-limited, when calculated on t...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1954
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136204/ https://www.ncbi.nlm.nih.gov/pubmed/13130797 |
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author | Sagik, Bernard Puck, Theodore Levine, Seymour |
author_facet | Sagik, Bernard Puck, Theodore Levine, Seymour |
author_sort | Sagik, Bernard |
collection | PubMed |
description | Each chick and human red cell contains approximately 300 sites capable of attaching influenza virus particles. These correspond to an area representing approximately 2 per cent of the red cell surface. Although the rate of attachment of PR8 to red cells is not diffusion-limited, when calculated on the basis of the total cell area, it does approach the theoretical maximum for interaction between the virus and the fraction of the cell area known to contain attachment sites. It is demonstrated that the virus attachment can initiate a spreading disturbance on the red cell membrane which extends over an area far exceeding that covered by the attached virus and which leads to the destruction of receptor sites. This process does not involve cyclic virus attachment, elution from the cell, and reattachment to another site. Practically all the receptor sites on a cell are destroyed before any virus is liberated into the medium. The spontaneous elution of virus from red cells within 24 hours at 37° C. requires a threshold value of at least 3 and less than 5 virus particles per cell. Parallelisms between the spontaneous elution reaction and the phenomenon of lysis-from-without in the bacteriophage system are demonstrated. |
format | Text |
id | pubmed-2136204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1954 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21362042008-04-17 QUANTITATIVE ASPECTS OF THE SPONTANEOUS ELUTION OF INFLUENZA VIRUS FROM RED CELLS Sagik, Bernard Puck, Theodore Levine, Seymour J Exp Med Article Each chick and human red cell contains approximately 300 sites capable of attaching influenza virus particles. These correspond to an area representing approximately 2 per cent of the red cell surface. Although the rate of attachment of PR8 to red cells is not diffusion-limited, when calculated on the basis of the total cell area, it does approach the theoretical maximum for interaction between the virus and the fraction of the cell area known to contain attachment sites. It is demonstrated that the virus attachment can initiate a spreading disturbance on the red cell membrane which extends over an area far exceeding that covered by the attached virus and which leads to the destruction of receptor sites. This process does not involve cyclic virus attachment, elution from the cell, and reattachment to another site. Practically all the receptor sites on a cell are destroyed before any virus is liberated into the medium. The spontaneous elution of virus from red cells within 24 hours at 37° C. requires a threshold value of at least 3 and less than 5 virus particles per cell. Parallelisms between the spontaneous elution reaction and the phenomenon of lysis-from-without in the bacteriophage system are demonstrated. The Rockefeller University Press 1954-02-28 /pmc/articles/PMC2136204/ /pubmed/13130797 Text en Copyright © Copyright, 1954, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Sagik, Bernard Puck, Theodore Levine, Seymour QUANTITATIVE ASPECTS OF THE SPONTANEOUS ELUTION OF INFLUENZA VIRUS FROM RED CELLS |
title | QUANTITATIVE ASPECTS OF THE SPONTANEOUS ELUTION OF INFLUENZA VIRUS FROM RED CELLS |
title_full | QUANTITATIVE ASPECTS OF THE SPONTANEOUS ELUTION OF INFLUENZA VIRUS FROM RED CELLS |
title_fullStr | QUANTITATIVE ASPECTS OF THE SPONTANEOUS ELUTION OF INFLUENZA VIRUS FROM RED CELLS |
title_full_unstemmed | QUANTITATIVE ASPECTS OF THE SPONTANEOUS ELUTION OF INFLUENZA VIRUS FROM RED CELLS |
title_short | QUANTITATIVE ASPECTS OF THE SPONTANEOUS ELUTION OF INFLUENZA VIRUS FROM RED CELLS |
title_sort | quantitative aspects of the spontaneous elution of influenza virus from red cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136204/ https://www.ncbi.nlm.nih.gov/pubmed/13130797 |
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