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Insertional Mutation of the Drosophila Nuclear Lamin Dm(0 )Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae
Nuclear lamins are thought to play an important role in disassembly and reassembly of the nucleus during mitosis. Here, we describe a Drosophila lamin Dm(0) mutant resulting from a P element insertion into the first intron of the Dm(0) gene. Homozygous mutant animals showed a severe phenotype includ...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136230/ https://www.ncbi.nlm.nih.gov/pubmed/9166402 |
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author | Lenz-Böhme, Bettina Wismar, Jasmine Fuchs, Silke Reifegerste, Rita Buchner, Erich Betz, Heinrich Schmitt, Bertram |
author_facet | Lenz-Böhme, Bettina Wismar, Jasmine Fuchs, Silke Reifegerste, Rita Buchner, Erich Betz, Heinrich Schmitt, Bertram |
author_sort | Lenz-Böhme, Bettina |
collection | PubMed |
description | Nuclear lamins are thought to play an important role in disassembly and reassembly of the nucleus during mitosis. Here, we describe a Drosophila lamin Dm(0) mutant resulting from a P element insertion into the first intron of the Dm(0) gene. Homozygous mutant animals showed a severe phenotype including retardation in development, reduced viability, sterility, and impaired locomotion. Immunocytochemical and ultrastructural analysis revealed that reduced lamin Dm(0) expression caused an enrichment of nuclear pore complexes in cytoplasmic annulate lamellae and in nuclear envelope clusters. In several cells, particularly the densely packed somata of the central nervous system, defective nuclear envelopes were observed in addition. All aspects of the mutant phenotype were rescued upon P element-mediated germline transformation with a lamin Dm(0) transgene. These data constitute the first genetic proof that lamins are essential for the structural organization of the cell nucleus. |
format | Text |
id | pubmed-2136230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21362302008-05-01 Insertional Mutation of the Drosophila Nuclear Lamin Dm(0 )Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae Lenz-Böhme, Bettina Wismar, Jasmine Fuchs, Silke Reifegerste, Rita Buchner, Erich Betz, Heinrich Schmitt, Bertram J Cell Biol Article Nuclear lamins are thought to play an important role in disassembly and reassembly of the nucleus during mitosis. Here, we describe a Drosophila lamin Dm(0) mutant resulting from a P element insertion into the first intron of the Dm(0) gene. Homozygous mutant animals showed a severe phenotype including retardation in development, reduced viability, sterility, and impaired locomotion. Immunocytochemical and ultrastructural analysis revealed that reduced lamin Dm(0) expression caused an enrichment of nuclear pore complexes in cytoplasmic annulate lamellae and in nuclear envelope clusters. In several cells, particularly the densely packed somata of the central nervous system, defective nuclear envelopes were observed in addition. All aspects of the mutant phenotype were rescued upon P element-mediated germline transformation with a lamin Dm(0) transgene. These data constitute the first genetic proof that lamins are essential for the structural organization of the cell nucleus. The Rockefeller University Press 1997-06-02 /pmc/articles/PMC2136230/ /pubmed/9166402 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Lenz-Böhme, Bettina Wismar, Jasmine Fuchs, Silke Reifegerste, Rita Buchner, Erich Betz, Heinrich Schmitt, Bertram Insertional Mutation of the Drosophila Nuclear Lamin Dm(0 )Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae |
title | Insertional Mutation of the Drosophila Nuclear Lamin Dm(0 )Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae |
title_full | Insertional Mutation of the Drosophila Nuclear Lamin Dm(0 )Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae |
title_fullStr | Insertional Mutation of the Drosophila Nuclear Lamin Dm(0 )Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae |
title_full_unstemmed | Insertional Mutation of the Drosophila Nuclear Lamin Dm(0 )Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae |
title_short | Insertional Mutation of the Drosophila Nuclear Lamin Dm(0 )Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae |
title_sort | insertional mutation of the drosophila nuclear lamin dm(0 )gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136230/ https://www.ncbi.nlm.nih.gov/pubmed/9166402 |
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