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IMMUNOCHEMICAL STUDIES OF ANTITOXIN PRODUCED IN NORMAL AND ALLERGIC INDIVIDUALS HYPERIMMUNIZED WITH DIPHTHERIA TOXOID : IV. DIFFERENCES BETWEEN HUMAN PRECIPITATING AND NON-PRECIPITATING SKIN-SENSITIZING DIPHTHERIA ANTITOXIN AS SHOWN BY ELECTROPHORESIS
Electrophoresis on a starch-supporting medium was used to fractionate sera containing human diphtheria antitoxin of the following varieties (a) precipitating antitoxin, (b) non-precipitating skin-sensitizing antitoxin, and (c) mixtures containing precipitating and skin-sensitizing antitoxins. Aliquo...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1954
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136346/ https://www.ncbi.nlm.nih.gov/pubmed/13163328 |
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author | Kuhns, William J. |
author_facet | Kuhns, William J. |
author_sort | Kuhns, William J. |
collection | PubMed |
description | Electrophoresis on a starch-supporting medium was used to fractionate sera containing human diphtheria antitoxin of the following varieties (a) precipitating antitoxin, (b) non-precipitating skin-sensitizing antitoxin, and (c) mixtures containing precipitating and skin-sensitizing antitoxins. Aliquots of the protein fractions thus separated were tested for activity using the rabbit toxin neutralization test, precipitin techniques, and passive transfer tests in human skin. Non-precipitating, skin-sensitizing diphtheria antitoxin migrated largely as a fast moving gamma (γ(1)) globulin. Passive transfer studies of isolated antitoxic fractions showed that they were as potent as whole serum in the ability to cause immediate wheal reactions. These fractions were not precipitable using appropriate quantities of purified toxoid. Precipitating diphtheria antitoxin migrated largely as a slow moving gamma (γ(2)) globulin. Isolated antitoxic fractions of appropriate strength obtained from representative sera were precipitable by toxin and were unable to cause immediate wheal reactions upon toxoid challenge in human recipients. Mixtures of skin-sensitizing and precipitating antitoxins were separable by the technique of starch electrophoresis. The individual components removed from mixtures by this method retained the properties by which they could be characterized in whole serum. |
format | Text |
id | pubmed-2136346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1954 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21363462008-04-17 IMMUNOCHEMICAL STUDIES OF ANTITOXIN PRODUCED IN NORMAL AND ALLERGIC INDIVIDUALS HYPERIMMUNIZED WITH DIPHTHERIA TOXOID : IV. DIFFERENCES BETWEEN HUMAN PRECIPITATING AND NON-PRECIPITATING SKIN-SENSITIZING DIPHTHERIA ANTITOXIN AS SHOWN BY ELECTROPHORESIS Kuhns, William J. J Exp Med Article Electrophoresis on a starch-supporting medium was used to fractionate sera containing human diphtheria antitoxin of the following varieties (a) precipitating antitoxin, (b) non-precipitating skin-sensitizing antitoxin, and (c) mixtures containing precipitating and skin-sensitizing antitoxins. Aliquots of the protein fractions thus separated were tested for activity using the rabbit toxin neutralization test, precipitin techniques, and passive transfer tests in human skin. Non-precipitating, skin-sensitizing diphtheria antitoxin migrated largely as a fast moving gamma (γ(1)) globulin. Passive transfer studies of isolated antitoxic fractions showed that they were as potent as whole serum in the ability to cause immediate wheal reactions. These fractions were not precipitable using appropriate quantities of purified toxoid. Precipitating diphtheria antitoxin migrated largely as a slow moving gamma (γ(2)) globulin. Isolated antitoxic fractions of appropriate strength obtained from representative sera were precipitable by toxin and were unable to cause immediate wheal reactions upon toxoid challenge in human recipients. Mixtures of skin-sensitizing and precipitating antitoxins were separable by the technique of starch electrophoresis. The individual components removed from mixtures by this method retained the properties by which they could be characterized in whole serum. The Rockefeller University Press 1954-06-01 /pmc/articles/PMC2136346/ /pubmed/13163328 Text en Copyright © Copyright, 1954, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Kuhns, William J. IMMUNOCHEMICAL STUDIES OF ANTITOXIN PRODUCED IN NORMAL AND ALLERGIC INDIVIDUALS HYPERIMMUNIZED WITH DIPHTHERIA TOXOID : IV. DIFFERENCES BETWEEN HUMAN PRECIPITATING AND NON-PRECIPITATING SKIN-SENSITIZING DIPHTHERIA ANTITOXIN AS SHOWN BY ELECTROPHORESIS |
title | IMMUNOCHEMICAL STUDIES OF ANTITOXIN PRODUCED IN NORMAL AND ALLERGIC INDIVIDUALS HYPERIMMUNIZED WITH DIPHTHERIA TOXOID : IV. DIFFERENCES BETWEEN HUMAN PRECIPITATING AND NON-PRECIPITATING SKIN-SENSITIZING DIPHTHERIA ANTITOXIN AS SHOWN BY ELECTROPHORESIS |
title_full | IMMUNOCHEMICAL STUDIES OF ANTITOXIN PRODUCED IN NORMAL AND ALLERGIC INDIVIDUALS HYPERIMMUNIZED WITH DIPHTHERIA TOXOID : IV. DIFFERENCES BETWEEN HUMAN PRECIPITATING AND NON-PRECIPITATING SKIN-SENSITIZING DIPHTHERIA ANTITOXIN AS SHOWN BY ELECTROPHORESIS |
title_fullStr | IMMUNOCHEMICAL STUDIES OF ANTITOXIN PRODUCED IN NORMAL AND ALLERGIC INDIVIDUALS HYPERIMMUNIZED WITH DIPHTHERIA TOXOID : IV. DIFFERENCES BETWEEN HUMAN PRECIPITATING AND NON-PRECIPITATING SKIN-SENSITIZING DIPHTHERIA ANTITOXIN AS SHOWN BY ELECTROPHORESIS |
title_full_unstemmed | IMMUNOCHEMICAL STUDIES OF ANTITOXIN PRODUCED IN NORMAL AND ALLERGIC INDIVIDUALS HYPERIMMUNIZED WITH DIPHTHERIA TOXOID : IV. DIFFERENCES BETWEEN HUMAN PRECIPITATING AND NON-PRECIPITATING SKIN-SENSITIZING DIPHTHERIA ANTITOXIN AS SHOWN BY ELECTROPHORESIS |
title_short | IMMUNOCHEMICAL STUDIES OF ANTITOXIN PRODUCED IN NORMAL AND ALLERGIC INDIVIDUALS HYPERIMMUNIZED WITH DIPHTHERIA TOXOID : IV. DIFFERENCES BETWEEN HUMAN PRECIPITATING AND NON-PRECIPITATING SKIN-SENSITIZING DIPHTHERIA ANTITOXIN AS SHOWN BY ELECTROPHORESIS |
title_sort | immunochemical studies of antitoxin produced in normal and allergic individuals hyperimmunized with diphtheria toxoid : iv. differences between human precipitating and non-precipitating skin-sensitizing diphtheria antitoxin as shown by electrophoresis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136346/ https://www.ncbi.nlm.nih.gov/pubmed/13163328 |
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