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STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE : I. HERPES SIMPLEX VIRUS
Herpes simplex virus was visualized by the electron microscope in sections of infected chorioallantoic membrane of chicken embryos. Removal of the embedding methacrylate from relatively thick sections permitted large numbers of viral particles to be seen but caused extensive alteration of cellular c...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1954
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136369/ https://www.ncbi.nlm.nih.gov/pubmed/13286423 |
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author | Morgan, Councilman Ellison, Solon A. Rose, Harry M. Moore, Dan H. |
author_facet | Morgan, Councilman Ellison, Solon A. Rose, Harry M. Moore, Dan H. |
author_sort | Morgan, Councilman |
collection | PubMed |
description | Herpes simplex virus was visualized by the electron microscope in sections of infected chorioallantoic membrane of chicken embryos. Removal of the embedding methacrylate from relatively thick sections permitted large numbers of viral particles to be seen but caused extensive alteration of cellular components as well as variable distortion of viral structure. This distortion was characterized by disruption of particles and loss of central bodies, resulting in ring or empty shell forms. An inner structure of the virus was revealed in ultrathin sections from which the embedding plastic was not removed. The nuclei of infected cells contained small, dense, primary bodies (30 to 40 mµ in diameter) as well as slightly larger and less dense particles (40 to 50 mµ in diameter) surrounded by a single membrane (70 to 100 mµ in diameter). In the cytoplasm most of the particles possessed a double outer membrane (120 to 130 mµ in diameter). It is suggested that the initial site of viral development is restricted to the nucleus where primary bodies form and become enclosed by a single outer membrane. Upon release into the cytoplasm these particles appear to acquire a second outer membrane and presumably represent the mature virus. |
format | Text |
id | pubmed-2136369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1954 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21363692008-04-17 STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE : I. HERPES SIMPLEX VIRUS Morgan, Councilman Ellison, Solon A. Rose, Harry M. Moore, Dan H. J Exp Med Article Herpes simplex virus was visualized by the electron microscope in sections of infected chorioallantoic membrane of chicken embryos. Removal of the embedding methacrylate from relatively thick sections permitted large numbers of viral particles to be seen but caused extensive alteration of cellular components as well as variable distortion of viral structure. This distortion was characterized by disruption of particles and loss of central bodies, resulting in ring or empty shell forms. An inner structure of the virus was revealed in ultrathin sections from which the embedding plastic was not removed. The nuclei of infected cells contained small, dense, primary bodies (30 to 40 mµ in diameter) as well as slightly larger and less dense particles (40 to 50 mµ in diameter) surrounded by a single membrane (70 to 100 mµ in diameter). In the cytoplasm most of the particles possessed a double outer membrane (120 to 130 mµ in diameter). It is suggested that the initial site of viral development is restricted to the nucleus where primary bodies form and become enclosed by a single outer membrane. Upon release into the cytoplasm these particles appear to acquire a second outer membrane and presumably represent the mature virus. The Rockefeller University Press 1954-08-01 /pmc/articles/PMC2136369/ /pubmed/13286423 Text en Copyright © Copyright, 1954, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Morgan, Councilman Ellison, Solon A. Rose, Harry M. Moore, Dan H. STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE : I. HERPES SIMPLEX VIRUS |
title | STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE : I. HERPES SIMPLEX VIRUS |
title_full | STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE : I. HERPES SIMPLEX VIRUS |
title_fullStr | STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE : I. HERPES SIMPLEX VIRUS |
title_full_unstemmed | STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE : I. HERPES SIMPLEX VIRUS |
title_short | STRUCTURE AND DEVELOPMENT OF VIRUSES AS OBSERVED IN THE ELECTRON MICROSCOPE : I. HERPES SIMPLEX VIRUS |
title_sort | structure and development of viruses as observed in the electron microscope : i. herpes simplex virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136369/ https://www.ncbi.nlm.nih.gov/pubmed/13286423 |
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