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VISUALIZATION OF EGYPT 101 VIRUS IN THE MOUSE'S BRAIN AND IN CULTURED HUMAN CARCINOMA CELLS BY MEANS OF FLUORESCENT ANTIBODY
The antigens of the Egypt virus have been detected by means of the fluorescent antibody technique in the neurons of the brain and spinal cord, in both sensory and motor areas, of infected mice. The antigens have also been observed in infected human epidermoid carcinoma cells in culture. In both case...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1955
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136515/ https://www.ncbi.nlm.nih.gov/pubmed/13252180 |
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author | Noyes, Wilbur Fiske |
author_facet | Noyes, Wilbur Fiske |
author_sort | Noyes, Wilbur Fiske |
collection | PubMed |
description | The antigens of the Egypt virus have been detected by means of the fluorescent antibody technique in the neurons of the brain and spinal cord, in both sensory and motor areas, of infected mice. The antigens have also been observed in infected human epidermoid carcinoma cells in culture. In both cases the antigens occurred in the cytoplasm of the cells exclusively. In the early stages of infection the antigen was localized about the nucleus of the human cultured cells. In some cases the staining of the antigens produced a sponge-like effect, probably because of the numerous vacuoles of lipid material. The antigens were observed in the protoplasmic connections between cells and they probably served as a main route of spread of the agent in these human cell cultures. The number of cells initially infected and showing antigen was proportional to the amount of virus added, and titration with one substrain of cells proved much more sensitive than intracerebral titration in the mouse. |
format | Text |
id | pubmed-2136515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1955 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21365152008-04-17 VISUALIZATION OF EGYPT 101 VIRUS IN THE MOUSE'S BRAIN AND IN CULTURED HUMAN CARCINOMA CELLS BY MEANS OF FLUORESCENT ANTIBODY Noyes, Wilbur Fiske J Exp Med Article The antigens of the Egypt virus have been detected by means of the fluorescent antibody technique in the neurons of the brain and spinal cord, in both sensory and motor areas, of infected mice. The antigens have also been observed in infected human epidermoid carcinoma cells in culture. In both cases the antigens occurred in the cytoplasm of the cells exclusively. In the early stages of infection the antigen was localized about the nucleus of the human cultured cells. In some cases the staining of the antigens produced a sponge-like effect, probably because of the numerous vacuoles of lipid material. The antigens were observed in the protoplasmic connections between cells and they probably served as a main route of spread of the agent in these human cell cultures. The number of cells initially infected and showing antigen was proportional to the amount of virus added, and titration with one substrain of cells proved much more sensitive than intracerebral titration in the mouse. The Rockefeller University Press 1955-09-01 /pmc/articles/PMC2136515/ /pubmed/13252180 Text en Copyright © Copyright, 1955, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Noyes, Wilbur Fiske VISUALIZATION OF EGYPT 101 VIRUS IN THE MOUSE'S BRAIN AND IN CULTURED HUMAN CARCINOMA CELLS BY MEANS OF FLUORESCENT ANTIBODY |
title | VISUALIZATION OF EGYPT 101 VIRUS IN THE MOUSE'S BRAIN AND IN CULTURED HUMAN CARCINOMA CELLS BY MEANS OF FLUORESCENT ANTIBODY |
title_full | VISUALIZATION OF EGYPT 101 VIRUS IN THE MOUSE'S BRAIN AND IN CULTURED HUMAN CARCINOMA CELLS BY MEANS OF FLUORESCENT ANTIBODY |
title_fullStr | VISUALIZATION OF EGYPT 101 VIRUS IN THE MOUSE'S BRAIN AND IN CULTURED HUMAN CARCINOMA CELLS BY MEANS OF FLUORESCENT ANTIBODY |
title_full_unstemmed | VISUALIZATION OF EGYPT 101 VIRUS IN THE MOUSE'S BRAIN AND IN CULTURED HUMAN CARCINOMA CELLS BY MEANS OF FLUORESCENT ANTIBODY |
title_short | VISUALIZATION OF EGYPT 101 VIRUS IN THE MOUSE'S BRAIN AND IN CULTURED HUMAN CARCINOMA CELLS BY MEANS OF FLUORESCENT ANTIBODY |
title_sort | visualization of egypt 101 virus in the mouse's brain and in cultured human carcinoma cells by means of fluorescent antibody |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136515/ https://www.ncbi.nlm.nih.gov/pubmed/13252180 |
work_keys_str_mv | AT noyeswilburfiske visualizationofegypt101virusinthemousesbrainandinculturedhumancarcinomacellsbymeansoffluorescentantibody |