NEPHROTOXIC SERUM NEPHRITIS IN RATS : II. PREPARATION AND CHARACTERIZATION OF A SOLUBLE PROTECTIVE FACTOR PRODUCED BY TRYPSIN DIGESTION OF RAT TISSUE HOMOGENATES

A soluble protective factor, capable of absorbing nephrotoxic antibodies from anti-rat kidney serum, can be obtained in high concentration by tryptic digestion of rat kidney homogenate. The factor is no longer antigenic or at most only slightly so. It is stable at 60°, destroyed by boiling, is non-d...

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Detalles Bibliográficos
Autores principales: Goodman, Howard C., Baxter, James H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1956
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136603/
https://www.ncbi.nlm.nih.gov/pubmed/13367325
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author Goodman, Howard C.
Baxter, James H.
author_facet Goodman, Howard C.
Baxter, James H.
author_sort Goodman, Howard C.
collection PubMed
description A soluble protective factor, capable of absorbing nephrotoxic antibodies from anti-rat kidney serum, can be obtained in high concentration by tryptic digestion of rat kidney homogenate. The factor is no longer antigenic or at most only slightly so. It is stable at 60°, destroyed by boiling, is non-dialyzable, can be precipitated by ammonium sulfate, but resists destruction by proteolytic and other enzymes. In accord with previous studies on the organ localization of the rat tissue antigen responsible for the production of nephrotoxic antibodies, the soluble protective factor, or antigen derivative, can be obtained by tryptic digestion of rat lung, skeletal muscle, heart, and liver.
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spelling pubmed-21366032008-04-17 NEPHROTOXIC SERUM NEPHRITIS IN RATS : II. PREPARATION AND CHARACTERIZATION OF A SOLUBLE PROTECTIVE FACTOR PRODUCED BY TRYPSIN DIGESTION OF RAT TISSUE HOMOGENATES Goodman, Howard C. Baxter, James H. J Exp Med Article A soluble protective factor, capable of absorbing nephrotoxic antibodies from anti-rat kidney serum, can be obtained in high concentration by tryptic digestion of rat kidney homogenate. The factor is no longer antigenic or at most only slightly so. It is stable at 60°, destroyed by boiling, is non-dialyzable, can be precipitated by ammonium sulfate, but resists destruction by proteolytic and other enzymes. In accord with previous studies on the organ localization of the rat tissue antigen responsible for the production of nephrotoxic antibodies, the soluble protective factor, or antigen derivative, can be obtained by tryptic digestion of rat lung, skeletal muscle, heart, and liver. The Rockefeller University Press 1956-09-30 /pmc/articles/PMC2136603/ /pubmed/13367325 Text en Copyright © Copyright, 1956, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Goodman, Howard C.
Baxter, James H.
NEPHROTOXIC SERUM NEPHRITIS IN RATS : II. PREPARATION AND CHARACTERIZATION OF A SOLUBLE PROTECTIVE FACTOR PRODUCED BY TRYPSIN DIGESTION OF RAT TISSUE HOMOGENATES
title NEPHROTOXIC SERUM NEPHRITIS IN RATS : II. PREPARATION AND CHARACTERIZATION OF A SOLUBLE PROTECTIVE FACTOR PRODUCED BY TRYPSIN DIGESTION OF RAT TISSUE HOMOGENATES
title_full NEPHROTOXIC SERUM NEPHRITIS IN RATS : II. PREPARATION AND CHARACTERIZATION OF A SOLUBLE PROTECTIVE FACTOR PRODUCED BY TRYPSIN DIGESTION OF RAT TISSUE HOMOGENATES
title_fullStr NEPHROTOXIC SERUM NEPHRITIS IN RATS : II. PREPARATION AND CHARACTERIZATION OF A SOLUBLE PROTECTIVE FACTOR PRODUCED BY TRYPSIN DIGESTION OF RAT TISSUE HOMOGENATES
title_full_unstemmed NEPHROTOXIC SERUM NEPHRITIS IN RATS : II. PREPARATION AND CHARACTERIZATION OF A SOLUBLE PROTECTIVE FACTOR PRODUCED BY TRYPSIN DIGESTION OF RAT TISSUE HOMOGENATES
title_short NEPHROTOXIC SERUM NEPHRITIS IN RATS : II. PREPARATION AND CHARACTERIZATION OF A SOLUBLE PROTECTIVE FACTOR PRODUCED BY TRYPSIN DIGESTION OF RAT TISSUE HOMOGENATES
title_sort nephrotoxic serum nephritis in rats : ii. preparation and characterization of a soluble protective factor produced by trypsin digestion of rat tissue homogenates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136603/
https://www.ncbi.nlm.nih.gov/pubmed/13367325
work_keys_str_mv AT goodmanhowardc nephrotoxicserumnephritisinratsiipreparationandcharacterizationofasolubleprotectivefactorproducedbytrypsindigestionofrattissuehomogenates
AT baxterjamesh nephrotoxicserumnephritisinratsiipreparationandcharacterizationofasolubleprotectivefactorproducedbytrypsindigestionofrattissuehomogenates

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