THE LOCALIZATION OF SKIN-SENSITIZING ANTIBODY IN THE SERA OF RAGWEED-SENSITIVE INDIVIDUALS BY ELECTROPHORESIS

Starch electrophoresis was used to separate allergic sera into electrophoretically distinct albumin, α-, β-, and γ-globulin fractions. The identity of the various fractions was established by free and paper electrophoresis. The γ-globulin fractions were also characterized by ultracentrifugation. The...

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Detalles Bibliográficos
Autores principales: Sehon, Alec H., Harter, John G., Rose, Bram
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1956
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136628/
https://www.ncbi.nlm.nih.gov/pubmed/13319586
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author Sehon, Alec H.
Harter, John G.
Rose, Bram
author_facet Sehon, Alec H.
Harter, John G.
Rose, Bram
author_sort Sehon, Alec H.
collection PubMed
description Starch electrophoresis was used to separate allergic sera into electrophoretically distinct albumin, α-, β-, and γ-globulin fractions. The identity of the various fractions was established by free and paper electrophoresis. The γ-globulin fractions were also characterized by ultracentrifugation. The serum skin-sensitizing antibody to ragweed pollen was shown to be associated with the γ-globulins in fourteen allergic sera by means of passive transfer technique. The mobility of the skin-sensitizing antibody corresponded to that of γ–1 and/or γ–2 globulins. All the other serum fractions were devoid of reaginic activity. Reaginic activity per se was not directly related to protein concentration. The discrepancies between these results, and those obtained by others and ourselves previously are discussed.
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spelling pubmed-21366282008-04-17 THE LOCALIZATION OF SKIN-SENSITIZING ANTIBODY IN THE SERA OF RAGWEED-SENSITIVE INDIVIDUALS BY ELECTROPHORESIS Sehon, Alec H. Harter, John G. Rose, Bram J Exp Med Article Starch electrophoresis was used to separate allergic sera into electrophoretically distinct albumin, α-, β-, and γ-globulin fractions. The identity of the various fractions was established by free and paper electrophoresis. The γ-globulin fractions were also characterized by ultracentrifugation. The serum skin-sensitizing antibody to ragweed pollen was shown to be associated with the γ-globulins in fourteen allergic sera by means of passive transfer technique. The mobility of the skin-sensitizing antibody corresponded to that of γ–1 and/or γ–2 globulins. All the other serum fractions were devoid of reaginic activity. Reaginic activity per se was not directly related to protein concentration. The discrepancies between these results, and those obtained by others and ourselves previously are discussed. The Rockefeller University Press 1956-05-01 /pmc/articles/PMC2136628/ /pubmed/13319586 Text en Copyright © Copyright, 1956, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Sehon, Alec H.
Harter, John G.
Rose, Bram
THE LOCALIZATION OF SKIN-SENSITIZING ANTIBODY IN THE SERA OF RAGWEED-SENSITIVE INDIVIDUALS BY ELECTROPHORESIS
title THE LOCALIZATION OF SKIN-SENSITIZING ANTIBODY IN THE SERA OF RAGWEED-SENSITIVE INDIVIDUALS BY ELECTROPHORESIS
title_full THE LOCALIZATION OF SKIN-SENSITIZING ANTIBODY IN THE SERA OF RAGWEED-SENSITIVE INDIVIDUALS BY ELECTROPHORESIS
title_fullStr THE LOCALIZATION OF SKIN-SENSITIZING ANTIBODY IN THE SERA OF RAGWEED-SENSITIVE INDIVIDUALS BY ELECTROPHORESIS
title_full_unstemmed THE LOCALIZATION OF SKIN-SENSITIZING ANTIBODY IN THE SERA OF RAGWEED-SENSITIVE INDIVIDUALS BY ELECTROPHORESIS
title_short THE LOCALIZATION OF SKIN-SENSITIZING ANTIBODY IN THE SERA OF RAGWEED-SENSITIVE INDIVIDUALS BY ELECTROPHORESIS
title_sort localization of skin-sensitizing antibody in the sera of ragweed-sensitive individuals by electrophoresis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136628/
https://www.ncbi.nlm.nih.gov/pubmed/13319586
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