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STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI : II. THE ACTION OF PHAGOCYTES UPON C(14)-LABELLED TUBERCLE BACILLI

Tubercle bacilli labelled with C(14) were prepared by growth on radioactive substrates such as glycerol, CO(2), and acetate. These organisms were exposed in vitro to leucocytes (mostly polymorphonuclear leucocytes) from peritoneal exudates of guinea pigs. The respiration of the leucocytes and of the...

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Autores principales: Stähelin, Hartmann, Karnovsky, Manfred L., Suter, Emanuel
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1956
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136642/
https://www.ncbi.nlm.nih.gov/pubmed/13332185
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author Stähelin, Hartmann
Karnovsky, Manfred L.
Suter, Emanuel
author_facet Stähelin, Hartmann
Karnovsky, Manfred L.
Suter, Emanuel
author_sort Stähelin, Hartmann
collection PubMed
description Tubercle bacilli labelled with C(14) were prepared by growth on radioactive substrates such as glycerol, CO(2), and acetate. These organisms were exposed in vitro to leucocytes (mostly polymorphonuclear leucocytes) from peritoneal exudates of guinea pigs. The respiration of the leucocytes and of the bacilli, alone and together, was followed by determining oxygen uptake and C(14)O(2) production. When heat-killed labelled tubercle bacilli were exposed to leucocytes there was little or no degradation of bacillary material to C(14)O(2) by leucocytic enzymes. On the other hand, conversion of components of sonically disrupted bacilli to C(14)O(2) by leucocytes was significant. It was possible to determine the oxygen uptake and C(14)O(2) production of phagocytized living tubercle bacilli, and it was found that after phagocytosis the bacilli maintained their rates of oxygen consumption and C(14)O(2) production. This finding was in contrast to observations made with Mycobacterium phlei, a saprophytic acid-fast organism, and with Bacillus subtilis. In these cases oxygen consumption and C(14)O(2) production declined after phagocytosis, and bacterial components were converted to carbon dioxide to a significant degree by leucocytic enzymes.
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spelling pubmed-21366422008-04-17 STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI : II. THE ACTION OF PHAGOCYTES UPON C(14)-LABELLED TUBERCLE BACILLI Stähelin, Hartmann Karnovsky, Manfred L. Suter, Emanuel J Exp Med Article Tubercle bacilli labelled with C(14) were prepared by growth on radioactive substrates such as glycerol, CO(2), and acetate. These organisms were exposed in vitro to leucocytes (mostly polymorphonuclear leucocytes) from peritoneal exudates of guinea pigs. The respiration of the leucocytes and of the bacilli, alone and together, was followed by determining oxygen uptake and C(14)O(2) production. When heat-killed labelled tubercle bacilli were exposed to leucocytes there was little or no degradation of bacillary material to C(14)O(2) by leucocytic enzymes. On the other hand, conversion of components of sonically disrupted bacilli to C(14)O(2) by leucocytes was significant. It was possible to determine the oxygen uptake and C(14)O(2) production of phagocytized living tubercle bacilli, and it was found that after phagocytosis the bacilli maintained their rates of oxygen consumption and C(14)O(2) production. This finding was in contrast to observations made with Mycobacterium phlei, a saprophytic acid-fast organism, and with Bacillus subtilis. In these cases oxygen consumption and C(14)O(2) production declined after phagocytosis, and bacterial components were converted to carbon dioxide to a significant degree by leucocytic enzymes. The Rockefeller University Press 1956-07-01 /pmc/articles/PMC2136642/ /pubmed/13332185 Text en Copyright © Copyright, 1956, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Stähelin, Hartmann
Karnovsky, Manfred L.
Suter, Emanuel
STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI : II. THE ACTION OF PHAGOCYTES UPON C(14)-LABELLED TUBERCLE BACILLI
title STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI : II. THE ACTION OF PHAGOCYTES UPON C(14)-LABELLED TUBERCLE BACILLI
title_full STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI : II. THE ACTION OF PHAGOCYTES UPON C(14)-LABELLED TUBERCLE BACILLI
title_fullStr STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI : II. THE ACTION OF PHAGOCYTES UPON C(14)-LABELLED TUBERCLE BACILLI
title_full_unstemmed STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI : II. THE ACTION OF PHAGOCYTES UPON C(14)-LABELLED TUBERCLE BACILLI
title_short STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI : II. THE ACTION OF PHAGOCYTES UPON C(14)-LABELLED TUBERCLE BACILLI
title_sort studies on the interaction between phagocytes and tubercle bacilli : ii. the action of phagocytes upon c(14)-labelled tubercle bacilli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136642/
https://www.ncbi.nlm.nih.gov/pubmed/13332185
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