BACTERICIDAL ACTIVITY OF RAT LEUCOCYTIC EXTRACTS : II. CHARACTERIZATION OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTE MITOCHONDRIAL EXTRACTS

The bactericidal substance present in mitochondrial extracts from rat leucocytes was found to be non-dialyzable, fractionated at 30 per cent (NH(4)SO(4) saturation and was able to withstand temperatures up to 100°C. The tentative identification of a lipoprotein as the responsible bactericidal substa...

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Detalles Bibliográficos
Autores principales: Fishman, M., Cole, L. J., Silverman, M. S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1957
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136713/
https://www.ncbi.nlm.nih.gov/pubmed/13428921
Descripción
Sumario:The bactericidal substance present in mitochondrial extracts from rat leucocytes was found to be non-dialyzable, fractionated at 30 per cent (NH(4)SO(4) saturation and was able to withstand temperatures up to 100°C. The tentative identification of a lipoprotein as the responsible bactericidal substance is based on various observations; an ultraviolet absorption at a peak associated with alpha, beta unsaturated fatty acids, 205 to 208 mµ, inactivation of the bactericidal substance by ether treatment at –70°C. but not at 0°C. and inhibition of the bactericidal activity with lipase treatment as well as with trypsin.

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