BACTERICIDAL ACTIVITY OF RAT LEUCOCYTIC EXTRACTS : II. CHARACTERIZATION OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTE MITOCHONDRIAL EXTRACTS

The bactericidal substance present in mitochondrial extracts from rat leucocytes was found to be non-dialyzable, fractionated at 30 per cent (NH(4)SO(4) saturation and was able to withstand temperatures up to 100°C. The tentative identification of a lipoprotein as the responsible bactericidal substa...

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Detalles Bibliográficos
Autores principales: Fishman, M., Cole, L. J., Silverman, M. S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1957
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136713/
https://www.ncbi.nlm.nih.gov/pubmed/13428921
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author Fishman, M.
Cole, L. J.
Silverman, M. S.
author_facet Fishman, M.
Cole, L. J.
Silverman, M. S.
author_sort Fishman, M.
collection PubMed
description The bactericidal substance present in mitochondrial extracts from rat leucocytes was found to be non-dialyzable, fractionated at 30 per cent (NH(4)SO(4) saturation and was able to withstand temperatures up to 100°C. The tentative identification of a lipoprotein as the responsible bactericidal substance is based on various observations; an ultraviolet absorption at a peak associated with alpha, beta unsaturated fatty acids, 205 to 208 mµ, inactivation of the bactericidal substance by ether treatment at –70°C. but not at 0°C. and inhibition of the bactericidal activity with lipase treatment as well as with trypsin.
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spelling pubmed-21367132008-04-17 BACTERICIDAL ACTIVITY OF RAT LEUCOCYTIC EXTRACTS : II. CHARACTERIZATION OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTE MITOCHONDRIAL EXTRACTS Fishman, M. Cole, L. J. Silverman, M. S. J Exp Med Article The bactericidal substance present in mitochondrial extracts from rat leucocytes was found to be non-dialyzable, fractionated at 30 per cent (NH(4)SO(4) saturation and was able to withstand temperatures up to 100°C. The tentative identification of a lipoprotein as the responsible bactericidal substance is based on various observations; an ultraviolet absorption at a peak associated with alpha, beta unsaturated fatty acids, 205 to 208 mµ, inactivation of the bactericidal substance by ether treatment at –70°C. but not at 0°C. and inhibition of the bactericidal activity with lipase treatment as well as with trypsin. The Rockefeller University Press 1957-06-01 /pmc/articles/PMC2136713/ /pubmed/13428921 Text en Copyright © Copyright, 1957, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Fishman, M.
Cole, L. J.
Silverman, M. S.
BACTERICIDAL ACTIVITY OF RAT LEUCOCYTIC EXTRACTS : II. CHARACTERIZATION OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTE MITOCHONDRIAL EXTRACTS
title BACTERICIDAL ACTIVITY OF RAT LEUCOCYTIC EXTRACTS : II. CHARACTERIZATION OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTE MITOCHONDRIAL EXTRACTS
title_full BACTERICIDAL ACTIVITY OF RAT LEUCOCYTIC EXTRACTS : II. CHARACTERIZATION OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTE MITOCHONDRIAL EXTRACTS
title_fullStr BACTERICIDAL ACTIVITY OF RAT LEUCOCYTIC EXTRACTS : II. CHARACTERIZATION OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTE MITOCHONDRIAL EXTRACTS
title_full_unstemmed BACTERICIDAL ACTIVITY OF RAT LEUCOCYTIC EXTRACTS : II. CHARACTERIZATION OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTE MITOCHONDRIAL EXTRACTS
title_short BACTERICIDAL ACTIVITY OF RAT LEUCOCYTIC EXTRACTS : II. CHARACTERIZATION OF THE BACTERICIDAL SUBSTANCE IN LEUCOCYTE MITOCHONDRIAL EXTRACTS
title_sort bactericidal activity of rat leucocytic extracts : ii. characterization of the bactericidal substance in leucocyte mitochondrial extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136713/
https://www.ncbi.nlm.nih.gov/pubmed/13428921
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AT colelj bactericidalactivityofratleucocyticextractsiicharacterizationofthebactericidalsubstanceinleucocytemitochondrialextracts
AT silvermanms bactericidalactivityofratleucocyticextractsiicharacterizationofthebactericidalsubstanceinleucocytemitochondrialextracts

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