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ON THE CONCENTRATION OF 5-HYDROXYTRYPTAMINE IN MAMMALIAN ENTEROCHROMAFFIN CELLS AND ITS RELEASE BY RESERPINE

The enterochromaffin cells of the guinea pig duodenum and 5-hydroxytryptamine incorporated into gelatin models gave, after formalin fixation, nearly identical reactions with methenamine silver, ferric-ferricyanide, and diazotized o-aminoazotoluene. Following formaldehyde fixation the indole reaction...

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Detalles Bibliográficos
Autores principales: Benditt, Earl P., Wong, Ruth L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1957
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136718/
https://www.ncbi.nlm.nih.gov/pubmed/13428919
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author Benditt, Earl P.
Wong, Ruth L.
author_facet Benditt, Earl P.
Wong, Ruth L.
author_sort Benditt, Earl P.
collection PubMed
description The enterochromaffin cells of the guinea pig duodenum and 5-hydroxytryptamine incorporated into gelatin models gave, after formalin fixation, nearly identical reactions with methenamine silver, ferric-ferricyanide, and diazotized o-aminoazotoluene. Following formaldehyde fixation the indole reaction with p-dimethylaminobenzaldehyde was negative in tissue enterochromaffin cells; similarly the indole reaction was negative after formaldehyde fixation of gelatin models. In the duodenum of the guinea pig 5-hydroxytryptamine appears to be largely, if not entirely, contained in the enterochromaffin cells. Its intracellular concentration estimated from relative cell volume and assay of concentration in the whole tissue, was not less than 5 mg./gm. of cells, and as judged by the intensity of staining reaction may have been as high as 10 to 20 mg./gm. of cells. Reserpine administered parenterally caused release of the chromogenic material of duodenal enterochromaffin cells and a parallel reduction in the amount of extractable 5-hydroxytryptamine.
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spelling pubmed-21367182008-04-17 ON THE CONCENTRATION OF 5-HYDROXYTRYPTAMINE IN MAMMALIAN ENTEROCHROMAFFIN CELLS AND ITS RELEASE BY RESERPINE Benditt, Earl P. Wong, Ruth L. J Exp Med Article The enterochromaffin cells of the guinea pig duodenum and 5-hydroxytryptamine incorporated into gelatin models gave, after formalin fixation, nearly identical reactions with methenamine silver, ferric-ferricyanide, and diazotized o-aminoazotoluene. Following formaldehyde fixation the indole reaction with p-dimethylaminobenzaldehyde was negative in tissue enterochromaffin cells; similarly the indole reaction was negative after formaldehyde fixation of gelatin models. In the duodenum of the guinea pig 5-hydroxytryptamine appears to be largely, if not entirely, contained in the enterochromaffin cells. Its intracellular concentration estimated from relative cell volume and assay of concentration in the whole tissue, was not less than 5 mg./gm. of cells, and as judged by the intensity of staining reaction may have been as high as 10 to 20 mg./gm. of cells. Reserpine administered parenterally caused release of the chromogenic material of duodenal enterochromaffin cells and a parallel reduction in the amount of extractable 5-hydroxytryptamine. The Rockefeller University Press 1957-06-01 /pmc/articles/PMC2136718/ /pubmed/13428919 Text en Copyright © Copyright, 1957, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Benditt, Earl P.
Wong, Ruth L.
ON THE CONCENTRATION OF 5-HYDROXYTRYPTAMINE IN MAMMALIAN ENTEROCHROMAFFIN CELLS AND ITS RELEASE BY RESERPINE
title ON THE CONCENTRATION OF 5-HYDROXYTRYPTAMINE IN MAMMALIAN ENTEROCHROMAFFIN CELLS AND ITS RELEASE BY RESERPINE
title_full ON THE CONCENTRATION OF 5-HYDROXYTRYPTAMINE IN MAMMALIAN ENTEROCHROMAFFIN CELLS AND ITS RELEASE BY RESERPINE
title_fullStr ON THE CONCENTRATION OF 5-HYDROXYTRYPTAMINE IN MAMMALIAN ENTEROCHROMAFFIN CELLS AND ITS RELEASE BY RESERPINE
title_full_unstemmed ON THE CONCENTRATION OF 5-HYDROXYTRYPTAMINE IN MAMMALIAN ENTEROCHROMAFFIN CELLS AND ITS RELEASE BY RESERPINE
title_short ON THE CONCENTRATION OF 5-HYDROXYTRYPTAMINE IN MAMMALIAN ENTEROCHROMAFFIN CELLS AND ITS RELEASE BY RESERPINE
title_sort on the concentration of 5-hydroxytryptamine in mammalian enterochromaffin cells and its release by reserpine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136718/
https://www.ncbi.nlm.nih.gov/pubmed/13428919
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