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FURTHER STUDIES ON THE CHEMICAL BASIS FOR SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL CARBOHYDRATE
Azoproteins prepared with p-aminophenyl-β-N-acetyl-glucosaminide react in precipitin tests with Group A streptococcal antisera. The reaction is nonreciprocal, and antisera to the azoprotein do not react with Group A carbohydrate. Phenyl-N-acetyl-glucosaminides inhibit the reaction of Group A carbohy...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1958
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136878/ https://www.ncbi.nlm.nih.gov/pubmed/13575668 |
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author | McCarty, Maclyn |
author_facet | McCarty, Maclyn |
author_sort | McCarty, Maclyn |
collection | PubMed |
description | Azoproteins prepared with p-aminophenyl-β-N-acetyl-glucosaminide react in precipitin tests with Group A streptococcal antisera. The reaction is nonreciprocal, and antisera to the azoprotein do not react with Group A carbohydrate. Phenyl-N-acetyl-glucosaminides inhibit the reaction of Group A carbohydrate with homologous antisera and with antisera to the azoprotein. The β-anomer is more effective as an inhibitor than the α-anomer. Formation by a soil bacillus of the enzyme which removes N-acetyl-glucosamine from Group A carbohydrate is induced by phenyl-β-N-acetyl-glucosaminide but not by the α-compound. The enzyme, like the glucosaminidase of emulsin, appears to be specific for β-glucosaminides. Neither the induced enzyme nor emulsin effectively remove all of the N-acetyl-glucosamine from the azoprotein antigens. The findings support the view that β-N-acetyl-glucosaminide side chains represent the major antigenic determinant of Group A streptococcal carbohydrate. |
format | Text |
id | pubmed-2136878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1958 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21368782008-04-17 FURTHER STUDIES ON THE CHEMICAL BASIS FOR SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL CARBOHYDRATE McCarty, Maclyn J Exp Med Article Azoproteins prepared with p-aminophenyl-β-N-acetyl-glucosaminide react in precipitin tests with Group A streptococcal antisera. The reaction is nonreciprocal, and antisera to the azoprotein do not react with Group A carbohydrate. Phenyl-N-acetyl-glucosaminides inhibit the reaction of Group A carbohydrate with homologous antisera and with antisera to the azoprotein. The β-anomer is more effective as an inhibitor than the α-anomer. Formation by a soil bacillus of the enzyme which removes N-acetyl-glucosamine from Group A carbohydrate is induced by phenyl-β-N-acetyl-glucosaminide but not by the α-compound. The enzyme, like the glucosaminidase of emulsin, appears to be specific for β-glucosaminides. Neither the induced enzyme nor emulsin effectively remove all of the N-acetyl-glucosamine from the azoprotein antigens. The findings support the view that β-N-acetyl-glucosaminide side chains represent the major antigenic determinant of Group A streptococcal carbohydrate. The Rockefeller University Press 1958-09-01 /pmc/articles/PMC2136878/ /pubmed/13575668 Text en Copyright © Copyright, 1958, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article McCarty, Maclyn FURTHER STUDIES ON THE CHEMICAL BASIS FOR SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL CARBOHYDRATE |
title | FURTHER STUDIES ON THE CHEMICAL BASIS FOR SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL CARBOHYDRATE |
title_full | FURTHER STUDIES ON THE CHEMICAL BASIS FOR SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL CARBOHYDRATE |
title_fullStr | FURTHER STUDIES ON THE CHEMICAL BASIS FOR SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL CARBOHYDRATE |
title_full_unstemmed | FURTHER STUDIES ON THE CHEMICAL BASIS FOR SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL CARBOHYDRATE |
title_short | FURTHER STUDIES ON THE CHEMICAL BASIS FOR SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL CARBOHYDRATE |
title_sort | further studies on the chemical basis for serological specificity of group a streptococcal carbohydrate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136878/ https://www.ncbi.nlm.nih.gov/pubmed/13575668 |
work_keys_str_mv | AT mccartymaclyn furtherstudiesonthechemicalbasisforserologicalspecificityofgroupastreptococcalcarbohydrate |