INFECTIVITY OF RIBONUCLEIC ACID FROM POLIOVIRUS IN HUMAN CELL MONOLAYERS

Ribonucleic acid prepared by the method of Gierer and Schramm from concentrated and partially purified types I and II polioviruses has been demonstrated to be infectious for HeLa and human amnion cells in monolayers. In areas of cytopathogenic action resulting from invasion of cells by RNA, intact p...

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Autores principales: Alexander, Hattie E., Koch, Gebhard, Mountain, Isabel Morgan, Van Damme, Olga
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136898/
https://www.ncbi.nlm.nih.gov/pubmed/13575680
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author Alexander, Hattie E.
Koch, Gebhard
Mountain, Isabel Morgan
Van Damme, Olga
author_facet Alexander, Hattie E.
Koch, Gebhard
Mountain, Isabel Morgan
Van Damme, Olga
author_sort Alexander, Hattie E.
collection PubMed
description Ribonucleic acid prepared by the method of Gierer and Schramm from concentrated and partially purified types I and II polioviruses has been demonstrated to be infectious for HeLa and human amnion cells in monolayers. In areas of cytopathogenic action resulting from invasion of cells by RNA, intact poliovirus, of the type from which the RNA had been prepared, is present. The infectivity of the RNA was completely inactivated by a 2 minute exposure to purified ribonuclease or to whole normal monkey serum shown to contain measurable concentrations of this enzyme. Whole virus infectivity was not influenced by RNAase or whole normal monkey serum. Normal and polio-immune globulin, desoxyribonuclease, lysozyme, proteolytic enzymes, and bovine albumin failed to inactivate the infectivity of RNA. The degree of infectivity of isolated RNA from poliovirus for cells in monolayer was greatly influenced by the ionic strength of the environment. The experimental evidence suggests that isolated poliovirus RNA is the carrier of the biological activity responsible for infection of cells and for transmission of genetic information which controls type specificity.
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spelling pubmed-21368982008-04-17 INFECTIVITY OF RIBONUCLEIC ACID FROM POLIOVIRUS IN HUMAN CELL MONOLAYERS Alexander, Hattie E. Koch, Gebhard Mountain, Isabel Morgan Van Damme, Olga J Exp Med Article Ribonucleic acid prepared by the method of Gierer and Schramm from concentrated and partially purified types I and II polioviruses has been demonstrated to be infectious for HeLa and human amnion cells in monolayers. In areas of cytopathogenic action resulting from invasion of cells by RNA, intact poliovirus, of the type from which the RNA had been prepared, is present. The infectivity of the RNA was completely inactivated by a 2 minute exposure to purified ribonuclease or to whole normal monkey serum shown to contain measurable concentrations of this enzyme. Whole virus infectivity was not influenced by RNAase or whole normal monkey serum. Normal and polio-immune globulin, desoxyribonuclease, lysozyme, proteolytic enzymes, and bovine albumin failed to inactivate the infectivity of RNA. The degree of infectivity of isolated RNA from poliovirus for cells in monolayer was greatly influenced by the ionic strength of the environment. The experimental evidence suggests that isolated poliovirus RNA is the carrier of the biological activity responsible for infection of cells and for transmission of genetic information which controls type specificity. The Rockefeller University Press 1958-09-30 /pmc/articles/PMC2136898/ /pubmed/13575680 Text en Copyright © Copyright, 1958, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Alexander, Hattie E.
Koch, Gebhard
Mountain, Isabel Morgan
Van Damme, Olga
INFECTIVITY OF RIBONUCLEIC ACID FROM POLIOVIRUS IN HUMAN CELL MONOLAYERS
title INFECTIVITY OF RIBONUCLEIC ACID FROM POLIOVIRUS IN HUMAN CELL MONOLAYERS
title_full INFECTIVITY OF RIBONUCLEIC ACID FROM POLIOVIRUS IN HUMAN CELL MONOLAYERS
title_fullStr INFECTIVITY OF RIBONUCLEIC ACID FROM POLIOVIRUS IN HUMAN CELL MONOLAYERS
title_full_unstemmed INFECTIVITY OF RIBONUCLEIC ACID FROM POLIOVIRUS IN HUMAN CELL MONOLAYERS
title_short INFECTIVITY OF RIBONUCLEIC ACID FROM POLIOVIRUS IN HUMAN CELL MONOLAYERS
title_sort infectivity of ribonucleic acid from poliovirus in human cell monolayers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136898/
https://www.ncbi.nlm.nih.gov/pubmed/13575680
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