CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS

Infectious tissue culture fluids of the majority of serotypes of adenovirus at low dilutions detach HeLa or KB cells from glass surfaces within a few hours after inoculation. A reproducible method for testing cell detachment was devised. The factor present in infectious tissue culture fluids and res...

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Detalles Bibliográficos
Autores principales: Rowe, Wallace P., Hartley, Janet W., Roizman, Bernard, Levy, Hilton B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136912/
https://www.ncbi.nlm.nih.gov/pubmed/13587853
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author Rowe, Wallace P.
Hartley, Janet W.
Roizman, Bernard
Levy, Hilton B.
author_facet Rowe, Wallace P.
Hartley, Janet W.
Roizman, Bernard
Levy, Hilton B.
author_sort Rowe, Wallace P.
collection PubMed
description Infectious tissue culture fluids of the majority of serotypes of adenovirus at low dilutions detach HeLa or KB cells from glass surfaces within a few hours after inoculation. A reproducible method for testing cell detachment was devised. The factor present in infectious tissue culture fluids and responsible for cell detachment is trypsin-sensitive and non-dialyzable; it is smaller and more resistant to the effect of heat or ultraviolet light than the infectious virus particle. Cell detachment activity was found to be temperature-dependent, and the cell-detaching titer of infectious tissue culture fluids was not affected by repeated exposure to HeLa cells. Inhibition of cell detachment by human or rabbit sera was observed only when other antibodies to adenovirus antigens were also present, but the antibody inhibiting cell detachment could not be correlated quantitatively with complement-fixing or homologous neutralizing antibody.
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spelling pubmed-21369122008-04-17 CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS Rowe, Wallace P. Hartley, Janet W. Roizman, Bernard Levy, Hilton B. J Exp Med Article Infectious tissue culture fluids of the majority of serotypes of adenovirus at low dilutions detach HeLa or KB cells from glass surfaces within a few hours after inoculation. A reproducible method for testing cell detachment was devised. The factor present in infectious tissue culture fluids and responsible for cell detachment is trypsin-sensitive and non-dialyzable; it is smaller and more resistant to the effect of heat or ultraviolet light than the infectious virus particle. Cell detachment activity was found to be temperature-dependent, and the cell-detaching titer of infectious tissue culture fluids was not affected by repeated exposure to HeLa cells. Inhibition of cell detachment by human or rabbit sera was observed only when other antibodies to adenovirus antigens were also present, but the antibody inhibiting cell detachment could not be correlated quantitatively with complement-fixing or homologous neutralizing antibody. The Rockefeller University Press 1958-10-31 /pmc/articles/PMC2136912/ /pubmed/13587853 Text en Copyright © Copyright, 1958, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rowe, Wallace P.
Hartley, Janet W.
Roizman, Bernard
Levy, Hilton B.
CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS
title CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS
title_full CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS
title_fullStr CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS
title_full_unstemmed CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS
title_short CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS
title_sort characterization of a factor formed in the course of adenovirus infection of tissue cultures causing detachment of cells from glass
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136912/
https://www.ncbi.nlm.nih.gov/pubmed/13587853
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