GENETICS OF SOMATIC MAMMALIAN CELLS : III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS

A methodology designed to eliminate mitotic inhibitor action and involving use of pretested fetal calf serum and careful pH and temperature control has been described by which cells from normal human and animal tissue can be maintained in active growth for long periods in vitro without development o...

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Detalles Bibliográficos
Autores principales: Puck, Theodore T., Cieciura, Steven J., Robinson, Arthur
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1958
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136918/
https://www.ncbi.nlm.nih.gov/pubmed/13598821
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author Puck, Theodore T.
Cieciura, Steven J.
Robinson, Arthur
author_facet Puck, Theodore T.
Cieciura, Steven J.
Robinson, Arthur
author_sort Puck, Theodore T.
collection PubMed
description A methodology designed to eliminate mitotic inhibitor action and involving use of pretested fetal calf serum and careful pH and temperature control has been described by which cells from normal human and animal tissue can be maintained in active growth for long periods in vitro without development of aneuploidy. By means of this procedure, it is possible reliably to establish cell cultures from minute skin biopsies which can be taken from any individual. Clones of mammalian cells with chromosomal markers have been isolated by this means from x-irradiated non-irradiated cell cultures. Application of these techniques to chromosome delineation in large numbers of human subjects; determination of chromosomal sex in patients; spontaneuos and induced genetic changes in somatic mammalian cells in vivo and in vitro; comparison of metabolic differences between normal and cancerous cells and other problems have been indicated.
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spelling pubmed-21369182008-04-17 GENETICS OF SOMATIC MAMMALIAN CELLS : III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS Puck, Theodore T. Cieciura, Steven J. Robinson, Arthur J Exp Med Article A methodology designed to eliminate mitotic inhibitor action and involving use of pretested fetal calf serum and careful pH and temperature control has been described by which cells from normal human and animal tissue can be maintained in active growth for long periods in vitro without development of aneuploidy. By means of this procedure, it is possible reliably to establish cell cultures from minute skin biopsies which can be taken from any individual. Clones of mammalian cells with chromosomal markers have been isolated by this means from x-irradiated non-irradiated cell cultures. Application of these techniques to chromosome delineation in large numbers of human subjects; determination of chromosomal sex in patients; spontaneuos and induced genetic changes in somatic mammalian cells in vivo and in vitro; comparison of metabolic differences between normal and cancerous cells and other problems have been indicated. The Rockefeller University Press 1958-11-30 /pmc/articles/PMC2136918/ /pubmed/13598821 Text en Copyright © Copyright, 1958, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Puck, Theodore T.
Cieciura, Steven J.
Robinson, Arthur
GENETICS OF SOMATIC MAMMALIAN CELLS : III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS
title GENETICS OF SOMATIC MAMMALIAN CELLS : III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS
title_full GENETICS OF SOMATIC MAMMALIAN CELLS : III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS
title_fullStr GENETICS OF SOMATIC MAMMALIAN CELLS : III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS
title_full_unstemmed GENETICS OF SOMATIC MAMMALIAN CELLS : III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS
title_short GENETICS OF SOMATIC MAMMALIAN CELLS : III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS
title_sort genetics of somatic mammalian cells : iii. long-term cultivation of euploid cells from human and animal subjects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136918/
https://www.ncbi.nlm.nih.gov/pubmed/13598821
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AT robinsonarthur geneticsofsomaticmammaliancellsiiilongtermcultivationofeuploidcellsfromhumanandanimalsubjects

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