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COMPARATIVE STUDIES ON THE PHYSICAL CHARACTERISTICS OF THE HERITABLE HAPTOGLOBIN GROUPS OF HUMAN SERUM

Ultracentrifugal studies of the α(2)-proteins of normal human serum before and after the addition of hemoglobin have revealed three separate and clearly distinguishable patterns based on the three major serum haptoglobin groups. Isolation of the three haptoglobin hemoglobin complexes disclosed chara...

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Autores principales: Bearn, A. G., Franklin, E. C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136930/
https://www.ncbi.nlm.nih.gov/pubmed/13611164
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author Bearn, A. G.
Franklin, E. C.
author_facet Bearn, A. G.
Franklin, E. C.
author_sort Bearn, A. G.
collection PubMed
description Ultracentrifugal studies of the α(2)-proteins of normal human serum before and after the addition of hemoglobin have revealed three separate and clearly distinguishable patterns based on the three major serum haptoglobin groups. Isolation of the three haptoglobin hemoglobin complexes disclosed characteristic patterns for each group. The heterozygote was found to possess electrophoretic and ultracentrifugal components not seen in either homozygote. Separation of normal human serum, to which hemoglobin had been added, by conventional starch zone electrophoresis resulted in three characteristic patterns which also permitted the ready identification of the serum haptoglobin group. The haptoglobin hemoglobin complex from Group 1-1 had the fastest mobility and that of Group 2-2 the slowest. The heterozygote Group 2-1 had an intermediate mobility. Immunologic differences between the haptoglobin hemoglobin complexes of the three groups could not be detected. Some of the variations in the reported electrophoretic and ultracentrifugal patterns of normal human serum could be reasonably ascribed to the group variation of the haptoglobins in man. The physical studies imply that the proteins characteristic of the heterozygote differ in size and charge from those present in either of the two homozygotes and indicate that the genetic control of the synthesis of the serum haptoglobins is probably exceedingly complex.
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spelling pubmed-21369302008-04-17 COMPARATIVE STUDIES ON THE PHYSICAL CHARACTERISTICS OF THE HERITABLE HAPTOGLOBIN GROUPS OF HUMAN SERUM Bearn, A. G. Franklin, E. C. J Exp Med Article Ultracentrifugal studies of the α(2)-proteins of normal human serum before and after the addition of hemoglobin have revealed three separate and clearly distinguishable patterns based on the three major serum haptoglobin groups. Isolation of the three haptoglobin hemoglobin complexes disclosed characteristic patterns for each group. The heterozygote was found to possess electrophoretic and ultracentrifugal components not seen in either homozygote. Separation of normal human serum, to which hemoglobin had been added, by conventional starch zone electrophoresis resulted in three characteristic patterns which also permitted the ready identification of the serum haptoglobin group. The haptoglobin hemoglobin complex from Group 1-1 had the fastest mobility and that of Group 2-2 the slowest. The heterozygote Group 2-1 had an intermediate mobility. Immunologic differences between the haptoglobin hemoglobin complexes of the three groups could not be detected. Some of the variations in the reported electrophoretic and ultracentrifugal patterns of normal human serum could be reasonably ascribed to the group variation of the haptoglobins in man. The physical studies imply that the proteins characteristic of the heterozygote differ in size and charge from those present in either of the two homozygotes and indicate that the genetic control of the synthesis of the serum haptoglobins is probably exceedingly complex. The Rockefeller University Press 1959-01-01 /pmc/articles/PMC2136930/ /pubmed/13611164 Text en Copyright © Copyright, 1959, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Bearn, A. G.
Franklin, E. C.
COMPARATIVE STUDIES ON THE PHYSICAL CHARACTERISTICS OF THE HERITABLE HAPTOGLOBIN GROUPS OF HUMAN SERUM
title COMPARATIVE STUDIES ON THE PHYSICAL CHARACTERISTICS OF THE HERITABLE HAPTOGLOBIN GROUPS OF HUMAN SERUM
title_full COMPARATIVE STUDIES ON THE PHYSICAL CHARACTERISTICS OF THE HERITABLE HAPTOGLOBIN GROUPS OF HUMAN SERUM
title_fullStr COMPARATIVE STUDIES ON THE PHYSICAL CHARACTERISTICS OF THE HERITABLE HAPTOGLOBIN GROUPS OF HUMAN SERUM
title_full_unstemmed COMPARATIVE STUDIES ON THE PHYSICAL CHARACTERISTICS OF THE HERITABLE HAPTOGLOBIN GROUPS OF HUMAN SERUM
title_short COMPARATIVE STUDIES ON THE PHYSICAL CHARACTERISTICS OF THE HERITABLE HAPTOGLOBIN GROUPS OF HUMAN SERUM
title_sort comparative studies on the physical characteristics of the heritable haptoglobin groups of human serum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136930/
https://www.ncbi.nlm.nih.gov/pubmed/13611164
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