INTRACELLULAR LOCALIZATION OF TYPE 4 ADENOVIRUS : I. CELLULAR FRACTIONATION STUDIES

HeLa cells infected with types 1 or 4 adenovirus were separated into cytoplasmic and nuclear fractions by mechanical disruption and differential centrifugation and the quantity of infectious virus in each was determined. The results showed that the majority of infectious virus of both types could be...

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Autores principales: Denny, Floyd W., Ginsberg, Harold S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136933/
https://www.ncbi.nlm.nih.gov/pubmed/13611165
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author Denny, Floyd W.
Ginsberg, Harold S.
author_facet Denny, Floyd W.
Ginsberg, Harold S.
author_sort Denny, Floyd W.
collection PubMed
description HeLa cells infected with types 1 or 4 adenovirus were separated into cytoplasmic and nuclear fractions by mechanical disruption and differential centrifugation and the quantity of infectious virus in each was determined. The results showed that the majority of infectious virus of both types could be isolated in the cytoplasmic fraction. It was not possible to explain the large amount of type 4 virus in the cytoplasmic fraction by the number of nuclei disrupted in the fractionation procedure, but the amount of type 1 virus in the cytoplasmic fraction could have been contributed by disrupted nuclei. This suggested that there might be a basic difference in the intracellular formation of the two types of virus. The intracellular distribution of complement-fixing antigen was similar to that of infectious virus in type 4-infected cells. Technical difficulties, inherent in cellular fractionation studies, were encountered but did not appear to explain the results obtained.
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spelling pubmed-21369332008-04-17 INTRACELLULAR LOCALIZATION OF TYPE 4 ADENOVIRUS : I. CELLULAR FRACTIONATION STUDIES Denny, Floyd W. Ginsberg, Harold S. J Exp Med Article HeLa cells infected with types 1 or 4 adenovirus were separated into cytoplasmic and nuclear fractions by mechanical disruption and differential centrifugation and the quantity of infectious virus in each was determined. The results showed that the majority of infectious virus of both types could be isolated in the cytoplasmic fraction. It was not possible to explain the large amount of type 4 virus in the cytoplasmic fraction by the number of nuclei disrupted in the fractionation procedure, but the amount of type 1 virus in the cytoplasmic fraction could have been contributed by disrupted nuclei. This suggested that there might be a basic difference in the intracellular formation of the two types of virus. The intracellular distribution of complement-fixing antigen was similar to that of infectious virus in type 4-infected cells. Technical difficulties, inherent in cellular fractionation studies, were encountered but did not appear to explain the results obtained. The Rockefeller University Press 1959-01-01 /pmc/articles/PMC2136933/ /pubmed/13611165 Text en Copyright © Copyright, 1959, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Denny, Floyd W.
Ginsberg, Harold S.
INTRACELLULAR LOCALIZATION OF TYPE 4 ADENOVIRUS : I. CELLULAR FRACTIONATION STUDIES
title INTRACELLULAR LOCALIZATION OF TYPE 4 ADENOVIRUS : I. CELLULAR FRACTIONATION STUDIES
title_full INTRACELLULAR LOCALIZATION OF TYPE 4 ADENOVIRUS : I. CELLULAR FRACTIONATION STUDIES
title_fullStr INTRACELLULAR LOCALIZATION OF TYPE 4 ADENOVIRUS : I. CELLULAR FRACTIONATION STUDIES
title_full_unstemmed INTRACELLULAR LOCALIZATION OF TYPE 4 ADENOVIRUS : I. CELLULAR FRACTIONATION STUDIES
title_short INTRACELLULAR LOCALIZATION OF TYPE 4 ADENOVIRUS : I. CELLULAR FRACTIONATION STUDIES
title_sort intracellular localization of type 4 adenovirus : i. cellular fractionation studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136933/
https://www.ncbi.nlm.nih.gov/pubmed/13611165
work_keys_str_mv AT dennyfloydw intracellularlocalizationoftype4adenovirusicellularfractionationstudies
AT ginsbergharolds intracellularlocalizationoftype4adenovirusicellularfractionationstudies

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