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DOUBLE INFECTIONS OF SINGLE CELLS WITH ECHO 7 AND COXSACKIE A9 VIRUSES

Virus particles derived from single cells infected with two enteroviruses have been studied. Evidence was obtained to indicate that phenotypic, but not genotypic, mixing occurs between Coxsackie A9 (CAP) and ECHO 7 (E7) viruses. Monkey kidney cultures in monolayer were doubly infected with high mult...

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Autores principales: Itoh, Heihachi, Melnick, Joseph L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136956/
https://www.ncbi.nlm.nih.gov/pubmed/13641564
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author Itoh, Heihachi
Melnick, Joseph L.
author_facet Itoh, Heihachi
Melnick, Joseph L.
author_sort Itoh, Heihachi
collection PubMed
description Virus particles derived from single cells infected with two enteroviruses have been studied. Evidence was obtained to indicate that phenotypic, but not genotypic, mixing occurs between Coxsackie A9 (CAP) and ECHO 7 (E7) viruses. Monkey kidney cultures in monolayer were doubly infected with high multiplicities of CA9 and E7 viruses. During the latent period, the infected cells were suspended, diluted, and distributed under oil into droplets each containing a single cell, as checked by microscopic observation. The virus particles released by individual cells into the microdrop were characterized in differential plaque neutralization tests. Fifteen per cent of the microdrops contained doubly neutralizable particles, 53 per cent yielded either CA9 or E7 particles, and 34 yielded particles of an intermediate character (deficits between 37 and 75 per cent). On passage, the doubly neutralizable particles yielded progeny of both parental types. All passage strains behaved like the corresponding parent strain as regards pathogenicity for newborn mice, which is to say that this property was limited to virus particles with CA9 antigenicity. Coxsackie A9 has a more rapid growth cycle than ECHO 7 in rhesus monkey kidney cell cultures, and a slower one in patas cultures. In rhesus, when E7 virus was added first, CA9 could be added up to 2 hours later, and still a significant number of cells yielded either CA9 or doubly neutralizable virus. The converse was observed in patas cells.
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spelling pubmed-21369562008-04-17 DOUBLE INFECTIONS OF SINGLE CELLS WITH ECHO 7 AND COXSACKIE A9 VIRUSES Itoh, Heihachi Melnick, Joseph L. J Exp Med Article Virus particles derived from single cells infected with two enteroviruses have been studied. Evidence was obtained to indicate that phenotypic, but not genotypic, mixing occurs between Coxsackie A9 (CAP) and ECHO 7 (E7) viruses. Monkey kidney cultures in monolayer were doubly infected with high multiplicities of CA9 and E7 viruses. During the latent period, the infected cells were suspended, diluted, and distributed under oil into droplets each containing a single cell, as checked by microscopic observation. The virus particles released by individual cells into the microdrop were characterized in differential plaque neutralization tests. Fifteen per cent of the microdrops contained doubly neutralizable particles, 53 per cent yielded either CA9 or E7 particles, and 34 yielded particles of an intermediate character (deficits between 37 and 75 per cent). On passage, the doubly neutralizable particles yielded progeny of both parental types. All passage strains behaved like the corresponding parent strain as regards pathogenicity for newborn mice, which is to say that this property was limited to virus particles with CA9 antigenicity. Coxsackie A9 has a more rapid growth cycle than ECHO 7 in rhesus monkey kidney cell cultures, and a slower one in patas cultures. In rhesus, when E7 virus was added first, CA9 could be added up to 2 hours later, and still a significant number of cells yielded either CA9 or doubly neutralizable virus. The converse was observed in patas cells. The Rockefeller University Press 1959-03-31 /pmc/articles/PMC2136956/ /pubmed/13641564 Text en Copyright © Copyright, 1959, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Itoh, Heihachi
Melnick, Joseph L.
DOUBLE INFECTIONS OF SINGLE CELLS WITH ECHO 7 AND COXSACKIE A9 VIRUSES
title DOUBLE INFECTIONS OF SINGLE CELLS WITH ECHO 7 AND COXSACKIE A9 VIRUSES
title_full DOUBLE INFECTIONS OF SINGLE CELLS WITH ECHO 7 AND COXSACKIE A9 VIRUSES
title_fullStr DOUBLE INFECTIONS OF SINGLE CELLS WITH ECHO 7 AND COXSACKIE A9 VIRUSES
title_full_unstemmed DOUBLE INFECTIONS OF SINGLE CELLS WITH ECHO 7 AND COXSACKIE A9 VIRUSES
title_short DOUBLE INFECTIONS OF SINGLE CELLS WITH ECHO 7 AND COXSACKIE A9 VIRUSES
title_sort double infections of single cells with echo 7 and coxsackie a9 viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136956/
https://www.ncbi.nlm.nih.gov/pubmed/13641564
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