INTRACELLULAR SURVIVAL OF STAPHYLOCOCCI

A tissue culture procedure is described which permits the quantitative evaluation of the intracellular survival of staphylococci within leucocytes. Staphylococcus aureus survived, but did not multiply, within neutrophils and monocytes of normal rabbits. The same was true of normal human blood leucoc...

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Detalles Bibliográficos
Autores principales: Kapral, Frank A., Shayegani, Mehdi Gh.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136963/
https://www.ncbi.nlm.nih.gov/pubmed/13664874
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author Kapral, Frank A.
Shayegani, Mehdi Gh.
author_facet Kapral, Frank A.
Shayegani, Mehdi Gh.
author_sort Kapral, Frank A.
collection PubMed
description A tissue culture procedure is described which permits the quantitative evaluation of the intracellular survival of staphylococci within leucocytes. Staphylococcus aureus survived, but did not multiply, within neutrophils and monocytes of normal rabbits. The same was true of normal human blood leucocytes. Staphylococcus albus on the other hand was destroyed by these cells under the same conditions. Rat monocytes destroyed S. aureus and S. albus with equal facility. Although most experiments were carried out in the presence of 50 µg. streptomycin/ml., similar results were obtained without the use of this antibiotic. The applications of the tissue culture procedure with regard to studies on virulence and immunity in staphylococcal disease are discussed.
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spelling pubmed-21369632008-04-17 INTRACELLULAR SURVIVAL OF STAPHYLOCOCCI Kapral, Frank A. Shayegani, Mehdi Gh. J Exp Med Article A tissue culture procedure is described which permits the quantitative evaluation of the intracellular survival of staphylococci within leucocytes. Staphylococcus aureus survived, but did not multiply, within neutrophils and monocytes of normal rabbits. The same was true of normal human blood leucocytes. Staphylococcus albus on the other hand was destroyed by these cells under the same conditions. Rat monocytes destroyed S. aureus and S. albus with equal facility. Although most experiments were carried out in the presence of 50 µg. streptomycin/ml., similar results were obtained without the use of this antibiotic. The applications of the tissue culture procedure with regard to studies on virulence and immunity in staphylococcal disease are discussed. The Rockefeller University Press 1959-07-01 /pmc/articles/PMC2136963/ /pubmed/13664874 Text en Copyright © Copyright, 1959, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kapral, Frank A.
Shayegani, Mehdi Gh.
INTRACELLULAR SURVIVAL OF STAPHYLOCOCCI
title INTRACELLULAR SURVIVAL OF STAPHYLOCOCCI
title_full INTRACELLULAR SURVIVAL OF STAPHYLOCOCCI
title_fullStr INTRACELLULAR SURVIVAL OF STAPHYLOCOCCI
title_full_unstemmed INTRACELLULAR SURVIVAL OF STAPHYLOCOCCI
title_short INTRACELLULAR SURVIVAL OF STAPHYLOCOCCI
title_sort intracellular survival of staphylococci
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136963/
https://www.ncbi.nlm.nih.gov/pubmed/13664874
work_keys_str_mv AT kapralfranka intracellularsurvivalofstaphylococci
AT shayeganimehdigh intracellularsurvivalofstaphylococci

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