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MOLECULAR GROWTH REQUIREMENTS OF SINGLE MAMMALIAN CELLS : III. QUANTITATIVE COLONIAL GROWTH OF SINGLE S3 CELLS IN A MEDIUM CONTAINING SYNTHETIC SMALL MOLECULAR CONSTITUENTS AND TWO PURIFIED PROTEIN FRACTIONS
Two purified serum protein fractions, fetuin and serum albumin, will replace whole or dialyzed serum in supporting the growth of single S3 HeLa cells in an otherwise chemically defined nutrient solution. In the serum-free medium, single S3 cells will form macroscopic colonies with essentially 100 pe...
Autores principales: | Fisher, Harold W., Puck, Theodore T., Sato, Gordon |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1959
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136981/ https://www.ncbi.nlm.nih.gov/pubmed/13654634 |
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