THE EFFECT OF ANTIGENIC STIMULATION ON INCORPORATION OF PHOSPHATE AND METHIONINE INTO PROTEINS OF ISOLATED LYMPH NODE CELLS

Isolated lymph node cells incorporate inorganic orthophosphate into a protein fraction. The phosphorylated product is a phosphoprotein. The rate of phosphate incorporation into phosphoprotein was determined in cells isolated from regional lymph nodes at varying times after antigen injection. The rat...

Descripción completa

Detalles Bibliográficos
Autores principales: Kern, Milton, Eisen, Herman N.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136988/
https://www.ncbi.nlm.nih.gov/pubmed/13673135
_version_ 1782143248341401600
author Kern, Milton
Eisen, Herman N.
author_facet Kern, Milton
Eisen, Herman N.
author_sort Kern, Milton
collection PubMed
description Isolated lymph node cells incorporate inorganic orthophosphate into a protein fraction. The phosphorylated product is a phosphoprotein. The rate of phosphate incorporation into phosphoprotein was determined in cells isolated from regional lymph nodes at varying times after antigen injection. The rate was unaltered on the 3rd day, but was enhanced on the 4th day after injection. Parallel results were obtained with L-methionine incorporation into the same gross protein fraction. Possible relationships between antibody formation and the observed enhancement in phosphate incorporation into phosphoprotein are discussed.
format Text
id pubmed-2136988
institution National Center for Biotechnology Information
language English
publishDate 1959
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21369882008-04-17 THE EFFECT OF ANTIGENIC STIMULATION ON INCORPORATION OF PHOSPHATE AND METHIONINE INTO PROTEINS OF ISOLATED LYMPH NODE CELLS Kern, Milton Eisen, Herman N. J Exp Med Article Isolated lymph node cells incorporate inorganic orthophosphate into a protein fraction. The phosphorylated product is a phosphoprotein. The rate of phosphate incorporation into phosphoprotein was determined in cells isolated from regional lymph nodes at varying times after antigen injection. The rate was unaltered on the 3rd day, but was enhanced on the 4th day after injection. Parallel results were obtained with L-methionine incorporation into the same gross protein fraction. Possible relationships between antibody formation and the observed enhancement in phosphate incorporation into phosphoprotein are discussed. The Rockefeller University Press 1959-08-01 /pmc/articles/PMC2136988/ /pubmed/13673135 Text en Copyright © Copyright, 1959, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kern, Milton
Eisen, Herman N.
THE EFFECT OF ANTIGENIC STIMULATION ON INCORPORATION OF PHOSPHATE AND METHIONINE INTO PROTEINS OF ISOLATED LYMPH NODE CELLS
title THE EFFECT OF ANTIGENIC STIMULATION ON INCORPORATION OF PHOSPHATE AND METHIONINE INTO PROTEINS OF ISOLATED LYMPH NODE CELLS
title_full THE EFFECT OF ANTIGENIC STIMULATION ON INCORPORATION OF PHOSPHATE AND METHIONINE INTO PROTEINS OF ISOLATED LYMPH NODE CELLS
title_fullStr THE EFFECT OF ANTIGENIC STIMULATION ON INCORPORATION OF PHOSPHATE AND METHIONINE INTO PROTEINS OF ISOLATED LYMPH NODE CELLS
title_full_unstemmed THE EFFECT OF ANTIGENIC STIMULATION ON INCORPORATION OF PHOSPHATE AND METHIONINE INTO PROTEINS OF ISOLATED LYMPH NODE CELLS
title_short THE EFFECT OF ANTIGENIC STIMULATION ON INCORPORATION OF PHOSPHATE AND METHIONINE INTO PROTEINS OF ISOLATED LYMPH NODE CELLS
title_sort effect of antigenic stimulation on incorporation of phosphate and methionine into proteins of isolated lymph node cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136988/
https://www.ncbi.nlm.nih.gov/pubmed/13673135
work_keys_str_mv AT kernmilton theeffectofantigenicstimulationonincorporationofphosphateandmethionineintoproteinsofisolatedlymphnodecells
AT eisenhermann theeffectofantigenicstimulationonincorporationofphosphateandmethionineintoproteinsofisolatedlymphnodecells
AT kernmilton effectofantigenicstimulationonincorporationofphosphateandmethionineintoproteinsofisolatedlymphnodecells
AT eisenhermann effectofantigenicstimulationonincorporationofphosphateandmethionineintoproteinsofisolatedlymphnodecells

Ejemplares similares