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THE PRESENCE OF PLASMA INHIBITORS DURING THE CRISIS PHENOMENON IN EXPERIMENTAL RELAPSING FEVER (BORRELIA NOVYI)

The intensity and duration of Borrelia novyi infection in rats depends upon the dose of spirochetes administered. The spirochetemia in response to an inoculum of 1 x 10(8) spirochetes/kg. characteristically reaches a peak of 4 to 5 million organisms per c.mm. of blood at about 72 hours. Resolution o...

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Detalles Bibliográficos
Autor principal: Calabi, Ornella
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1959
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137020/
https://www.ncbi.nlm.nih.gov/pubmed/13848161
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author Calabi, Ornella
author_facet Calabi, Ornella
author_sort Calabi, Ornella
collection PubMed
description The intensity and duration of Borrelia novyi infection in rats depends upon the dose of spirochetes administered. The spirochetemia in response to an inoculum of 1 x 10(8) spirochetes/kg. characteristically reaches a peak of 4 to 5 million organisms per c.mm. of blood at about 72 hours. Resolution of the primary infection occurs within 48 hours after the peak counts have been observed, with crisis ending at 100 to 120 hours after inoculation. Treatment of the acute infection, so standardized, by 10 ml./kg. of crisis plasma intraperitoneally delays the onset of the spirochetemia 76 to 140 hours, and reduces the maximal spirochetal count 10(–3) to 10(–6) that of the unmodified controls. This is evidence that inhibitor is present in the plasma at the time of crisis and plays a role in limiting the primary infection and subsequent relapses. The activity of crisis plasma is not destroyed by freezing, or after storage at 4°C. for 4 days. Fractions I + II + III and IV + V+ VI from crisis plasma, obtained by method 10 of Cohn et al., were also effective in suppressing the acute spirochetemia, but differed in duration and effectiveness. The first of these was about as potent as the undiluted whole plasma, and the second about as potent as plasma diluted 1:4.
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spelling pubmed-21370202008-04-17 THE PRESENCE OF PLASMA INHIBITORS DURING THE CRISIS PHENOMENON IN EXPERIMENTAL RELAPSING FEVER (BORRELIA NOVYI) Calabi, Ornella J Exp Med Article The intensity and duration of Borrelia novyi infection in rats depends upon the dose of spirochetes administered. The spirochetemia in response to an inoculum of 1 x 10(8) spirochetes/kg. characteristically reaches a peak of 4 to 5 million organisms per c.mm. of blood at about 72 hours. Resolution of the primary infection occurs within 48 hours after the peak counts have been observed, with crisis ending at 100 to 120 hours after inoculation. Treatment of the acute infection, so standardized, by 10 ml./kg. of crisis plasma intraperitoneally delays the onset of the spirochetemia 76 to 140 hours, and reduces the maximal spirochetal count 10(–3) to 10(–6) that of the unmodified controls. This is evidence that inhibitor is present in the plasma at the time of crisis and plays a role in limiting the primary infection and subsequent relapses. The activity of crisis plasma is not destroyed by freezing, or after storage at 4°C. for 4 days. Fractions I + II + III and IV + V+ VI from crisis plasma, obtained by method 10 of Cohn et al., were also effective in suppressing the acute spirochetemia, but differed in duration and effectiveness. The first of these was about as potent as the undiluted whole plasma, and the second about as potent as plasma diluted 1:4. The Rockefeller University Press 1959-10-31 /pmc/articles/PMC2137020/ /pubmed/13848161 Text en Copyright © Copyright, 1959, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Calabi, Ornella
THE PRESENCE OF PLASMA INHIBITORS DURING THE CRISIS PHENOMENON IN EXPERIMENTAL RELAPSING FEVER (BORRELIA NOVYI)
title THE PRESENCE OF PLASMA INHIBITORS DURING THE CRISIS PHENOMENON IN EXPERIMENTAL RELAPSING FEVER (BORRELIA NOVYI)
title_full THE PRESENCE OF PLASMA INHIBITORS DURING THE CRISIS PHENOMENON IN EXPERIMENTAL RELAPSING FEVER (BORRELIA NOVYI)
title_fullStr THE PRESENCE OF PLASMA INHIBITORS DURING THE CRISIS PHENOMENON IN EXPERIMENTAL RELAPSING FEVER (BORRELIA NOVYI)
title_full_unstemmed THE PRESENCE OF PLASMA INHIBITORS DURING THE CRISIS PHENOMENON IN EXPERIMENTAL RELAPSING FEVER (BORRELIA NOVYI)
title_short THE PRESENCE OF PLASMA INHIBITORS DURING THE CRISIS PHENOMENON IN EXPERIMENTAL RELAPSING FEVER (BORRELIA NOVYI)
title_sort presence of plasma inhibitors during the crisis phenomenon in experimental relapsing fever (borrelia novyi)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137020/
https://www.ncbi.nlm.nih.gov/pubmed/13848161
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