THE EFFECT OF DIPHTHERIA TOXIN ON THE METABOLISM OF HELA CELLS : II. EFFECT ON NUCLEIC ACID METABOLISM

The effect of toxin on the incorporation of uridine and thymidine into nucleic acids was investigated. P(32) orthophosphate incorporation into the nucleoside phosphate fraction was also investigated. No early effect of toxin on nucleic acid synthesis was evident and only a slight effect of toxin on...

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Detalles Bibliográficos
Autor principal: Strauss, Norman
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1960
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137219/
https://www.ncbi.nlm.nih.gov/pubmed/19867174
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author Strauss, Norman
author_facet Strauss, Norman
author_sort Strauss, Norman
collection PubMed
description The effect of toxin on the incorporation of uridine and thymidine into nucleic acids was investigated. P(32) orthophosphate incorporation into the nucleoside phosphate fraction was also investigated. No early effect of toxin on nucleic acid synthesis was evident and only a slight effect of toxin on nucleoside phosphate formation was found prior to 1.5 hours after toxin addition. Both these processes, however, were found to be sensitive to dinitrophenol. These results are interpreted as indicating that toxin does not act by a general uncoupling of oxidation from phosphorylation. Other possible mechanisms of toxin action are proposed.
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spelling pubmed-21372192008-04-17 THE EFFECT OF DIPHTHERIA TOXIN ON THE METABOLISM OF HELA CELLS : II. EFFECT ON NUCLEIC ACID METABOLISM Strauss, Norman J Exp Med Article The effect of toxin on the incorporation of uridine and thymidine into nucleic acids was investigated. P(32) orthophosphate incorporation into the nucleoside phosphate fraction was also investigated. No early effect of toxin on nucleic acid synthesis was evident and only a slight effect of toxin on nucleoside phosphate formation was found prior to 1.5 hours after toxin addition. Both these processes, however, were found to be sensitive to dinitrophenol. These results are interpreted as indicating that toxin does not act by a general uncoupling of oxidation from phosphorylation. Other possible mechanisms of toxin action are proposed. The Rockefeller University Press 1960-08-01 /pmc/articles/PMC2137219/ /pubmed/19867174 Text en Copyright © Copyright, 1960, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Strauss, Norman
THE EFFECT OF DIPHTHERIA TOXIN ON THE METABOLISM OF HELA CELLS : II. EFFECT ON NUCLEIC ACID METABOLISM
title THE EFFECT OF DIPHTHERIA TOXIN ON THE METABOLISM OF HELA CELLS : II. EFFECT ON NUCLEIC ACID METABOLISM
title_full THE EFFECT OF DIPHTHERIA TOXIN ON THE METABOLISM OF HELA CELLS : II. EFFECT ON NUCLEIC ACID METABOLISM
title_fullStr THE EFFECT OF DIPHTHERIA TOXIN ON THE METABOLISM OF HELA CELLS : II. EFFECT ON NUCLEIC ACID METABOLISM
title_full_unstemmed THE EFFECT OF DIPHTHERIA TOXIN ON THE METABOLISM OF HELA CELLS : II. EFFECT ON NUCLEIC ACID METABOLISM
title_short THE EFFECT OF DIPHTHERIA TOXIN ON THE METABOLISM OF HELA CELLS : II. EFFECT ON NUCLEIC ACID METABOLISM
title_sort effect of diphtheria toxin on the metabolism of hela cells : ii. effect on nucleic acid metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137219/
https://www.ncbi.nlm.nih.gov/pubmed/19867174
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