ON THE ROLE OF RIBONUCLEIC ACID IN ANIMAL VIRUS SYNTHESIS : II. STUDIES WITH RIBONUCLEASE

Ribonuclease is a highly active inhibitor of vaccinia virus multiplication in vitro in the chorioallantoic membrane removed from embryonated chicken eggs. It is also a highly active inhibitor of pock formation by vaccinia and herpes simplex viruses on the chorioallantoic membrane in vivo. Marked inh...

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Detalles Bibliográficos
Autores principales: Tamm, Igor, Bablanian, Rostom
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1960
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137258/
https://www.ncbi.nlm.nih.gov/pubmed/13775110
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author Tamm, Igor
Bablanian, Rostom
author_facet Tamm, Igor
Bablanian, Rostom
author_sort Tamm, Igor
collection PubMed
description Ribonuclease is a highly active inhibitor of vaccinia virus multiplication in vitro in the chorioallantoic membrane removed from embryonated chicken eggs. It is also a highly active inhibitor of pock formation by vaccinia and herpes simplex viruses on the chorioallantoic membrane in vivo. Marked inhibitory effects were obtained with 12.5 µg. of RNase. However, complete inhibition was not obtained with several hundred micrograms of the enzyme. RNase caused no inactivation of the infectivity of vaccinia virus particles but it had a marked inhibitory effect on multiplication of this virus when administered many hours after infection of host cells had occurred. RNase also failed to inactivate the infectivity of herpes simplex virus particles. The results obtained indicate that ribonucleic acid is necessary for the multiplication of two DNA-containing viruses; i.e., vaccinia and herpes simplex.
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spelling pubmed-21372582008-04-17 ON THE ROLE OF RIBONUCLEIC ACID IN ANIMAL VIRUS SYNTHESIS : II. STUDIES WITH RIBONUCLEASE Tamm, Igor Bablanian, Rostom J Exp Med Article Ribonuclease is a highly active inhibitor of vaccinia virus multiplication in vitro in the chorioallantoic membrane removed from embryonated chicken eggs. It is also a highly active inhibitor of pock formation by vaccinia and herpes simplex viruses on the chorioallantoic membrane in vivo. Marked inhibitory effects were obtained with 12.5 µg. of RNase. However, complete inhibition was not obtained with several hundred micrograms of the enzyme. RNase caused no inactivation of the infectivity of vaccinia virus particles but it had a marked inhibitory effect on multiplication of this virus when administered many hours after infection of host cells had occurred. RNase also failed to inactivate the infectivity of herpes simplex virus particles. The results obtained indicate that ribonucleic acid is necessary for the multiplication of two DNA-containing viruses; i.e., vaccinia and herpes simplex. The Rockefeller University Press 1960-02-29 /pmc/articles/PMC2137258/ /pubmed/13775110 Text en ©Copyright 1960, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Tamm, Igor
Bablanian, Rostom
ON THE ROLE OF RIBONUCLEIC ACID IN ANIMAL VIRUS SYNTHESIS : II. STUDIES WITH RIBONUCLEASE
title ON THE ROLE OF RIBONUCLEIC ACID IN ANIMAL VIRUS SYNTHESIS : II. STUDIES WITH RIBONUCLEASE
title_full ON THE ROLE OF RIBONUCLEIC ACID IN ANIMAL VIRUS SYNTHESIS : II. STUDIES WITH RIBONUCLEASE
title_fullStr ON THE ROLE OF RIBONUCLEIC ACID IN ANIMAL VIRUS SYNTHESIS : II. STUDIES WITH RIBONUCLEASE
title_full_unstemmed ON THE ROLE OF RIBONUCLEIC ACID IN ANIMAL VIRUS SYNTHESIS : II. STUDIES WITH RIBONUCLEASE
title_short ON THE ROLE OF RIBONUCLEIC ACID IN ANIMAL VIRUS SYNTHESIS : II. STUDIES WITH RIBONUCLEASE
title_sort on the role of ribonucleic acid in animal virus synthesis : ii. studies with ribonuclease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137258/
https://www.ncbi.nlm.nih.gov/pubmed/13775110
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AT bablanianrostom ontheroleofribonucleicacidinanimalvirussynthesisiistudieswithribonuclease

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