THE DEVELOPMENT OF ANTIBODIES TO PENICILLIN IN RABBITS

A method for the production of antibodies specifically directed against penicillin is described. The inability of this antibody to significantly reduce the antibiotic activity of penicillin is noted. Evidence to show the variability of specificities of various sera, some directed for the most part a...

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Detalles Bibliográficos
Autor principal: Josephson, Alan S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1960
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137283/
https://www.ncbi.nlm.nih.gov/pubmed/13790762
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author Josephson, Alan S.
author_facet Josephson, Alan S.
author_sort Josephson, Alan S.
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description A method for the production of antibodies specifically directed against penicillin is described. The inability of this antibody to significantly reduce the antibiotic activity of penicillin is noted. Evidence to show the variability of specificities of various sera, some directed for the most part against the side chain, others against the nucleus is presented. Studies on serum fractions separated electrophorectically indicate that the antibody migrates in the fast γ-globulin and β-globulin fractions and requires dextran or albumin to effect agglutination. The inability of penicilloic acid, an hydrolysis product of penicillin to provoke antibody formation despite its ability to inhibit the antibody is shown and the implications of this observations are discussed.
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spelling pubmed-21372832008-04-17 THE DEVELOPMENT OF ANTIBODIES TO PENICILLIN IN RABBITS Josephson, Alan S. J Exp Med Article A method for the production of antibodies specifically directed against penicillin is described. The inability of this antibody to significantly reduce the antibiotic activity of penicillin is noted. Evidence to show the variability of specificities of various sera, some directed for the most part against the side chain, others against the nucleus is presented. Studies on serum fractions separated electrophorectically indicate that the antibody migrates in the fast γ-globulin and β-globulin fractions and requires dextran or albumin to effect agglutination. The inability of penicilloic acid, an hydrolysis product of penicillin to provoke antibody formation despite its ability to inhibit the antibody is shown and the implications of this observations are discussed. The Rockefeller University Press 1960-05-01 /pmc/articles/PMC2137283/ /pubmed/13790762 Text en ©Copyright 1960, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Josephson, Alan S.
THE DEVELOPMENT OF ANTIBODIES TO PENICILLIN IN RABBITS
title THE DEVELOPMENT OF ANTIBODIES TO PENICILLIN IN RABBITS
title_full THE DEVELOPMENT OF ANTIBODIES TO PENICILLIN IN RABBITS
title_fullStr THE DEVELOPMENT OF ANTIBODIES TO PENICILLIN IN RABBITS
title_full_unstemmed THE DEVELOPMENT OF ANTIBODIES TO PENICILLIN IN RABBITS
title_short THE DEVELOPMENT OF ANTIBODIES TO PENICILLIN IN RABBITS
title_sort development of antibodies to penicillin in rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137283/
https://www.ncbi.nlm.nih.gov/pubmed/13790762
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