THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP

Injection of rabbits with Freund's complete adjuvant containing bovine γ-globulin substituted to near saturation with dinitrophenyl groups leads to the production of large amounts of antibodies specific for the 2,4-dinitrophenyl substituent. These antibodies can be isolated from sera in satisfa...

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Detalles Bibliográficos
Autores principales: Farah, Fuad S., Kern, Milton, Eisen, Herman N.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1960
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137313/
https://www.ncbi.nlm.nih.gov/pubmed/13698150
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author Farah, Fuad S.
Kern, Milton
Eisen, Herman N.
author_facet Farah, Fuad S.
Kern, Milton
Eisen, Herman N.
author_sort Farah, Fuad S.
collection PubMed
description Injection of rabbits with Freund's complete adjuvant containing bovine γ-globulin substituted to near saturation with dinitrophenyl groups leads to the production of large amounts of antibodies specific for the 2,4-dinitrophenyl substituent. These antibodies can be isolated from sera in satisfactory yield (40 per cent) and in a high state of purity (90 per cent specifically precipitable). Hapten dissolution, which was used to screen haptens for their potential usefulness as specific reagents in the purification procedure, provides a means for evaluating, in relative terms, antibody-hapten affinities (in competition with antigen).
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spelling pubmed-21373132008-04-17 THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP Farah, Fuad S. Kern, Milton Eisen, Herman N. J Exp Med Article Injection of rabbits with Freund's complete adjuvant containing bovine γ-globulin substituted to near saturation with dinitrophenyl groups leads to the production of large amounts of antibodies specific for the 2,4-dinitrophenyl substituent. These antibodies can be isolated from sera in satisfactory yield (40 per cent) and in a high state of purity (90 per cent specifically precipitable). Hapten dissolution, which was used to screen haptens for their potential usefulness as specific reagents in the purification procedure, provides a means for evaluating, in relative terms, antibody-hapten affinities (in competition with antigen). The Rockefeller University Press 1960-11-30 /pmc/articles/PMC2137313/ /pubmed/13698150 Text en Copyright © Copyright, 1960, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Farah, Fuad S.
Kern, Milton
Eisen, Herman N.
THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP
title THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP
title_full THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP
title_fullStr THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP
title_full_unstemmed THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP
title_short THE PREPARATION AND SOME PROPERTIES OF PURIFIED ANTIBODY SPECIFIC FOR THE 2,4-DINITROPHENYL GROUP
title_sort preparation and some properties of purified antibody specific for the 2,4-dinitrophenyl group
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137313/
https://www.ncbi.nlm.nih.gov/pubmed/13698150
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