STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT

A model system for the investigation of immune cellular injury in primary isolates of a fixed-tissue cell of human origin has been described, using metabolism-inhibition and uptake of trypan blue as independent criteria of cytoxicity. Cytotoxic effects on human amnion cells were produced by the comb...

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Detalles Bibliográficos
Autores principales: Ross, Ann, Lepow, Irwin H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1960
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137319/
https://www.ncbi.nlm.nih.gov/pubmed/13743575
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author Ross, Ann
Lepow, Irwin H.
author_facet Ross, Ann
Lepow, Irwin H.
author_sort Ross, Ann
collection PubMed
description A model system for the investigation of immune cellular injury in primary isolates of a fixed-tissue cell of human origin has been described, using metabolism-inhibition and uptake of trypan blue as independent criteria of cytoxicity. Cytotoxic effects on human amnion cells were produced by the combination of specific rabbit antibody and factors in normal human serum which were indistinguishable from the components of hemolytic complement and calcium and magnesium ions. The data have been discussed in relation to the apparent multiplicity of serologic systems which can effect cellular injury. The nature of the cell and the source of antibody or normal serum constituents have been emphasized as factors which may influence the experimental selection of a given humoral mechanism.
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spelling pubmed-21373192008-04-17 STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT Ross, Ann Lepow, Irwin H. J Exp Med Article A model system for the investigation of immune cellular injury in primary isolates of a fixed-tissue cell of human origin has been described, using metabolism-inhibition and uptake of trypan blue as independent criteria of cytoxicity. Cytotoxic effects on human amnion cells were produced by the combination of specific rabbit antibody and factors in normal human serum which were indistinguishable from the components of hemolytic complement and calcium and magnesium ions. The data have been discussed in relation to the apparent multiplicity of serologic systems which can effect cellular injury. The nature of the cell and the source of antibody or normal serum constituents have been emphasized as factors which may influence the experimental selection of a given humoral mechanism. The Rockefeller University Press 1960-11-30 /pmc/articles/PMC2137319/ /pubmed/13743575 Text en Copyright © Copyright, 1960, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Ross, Ann
Lepow, Irwin H.
STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT
title STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT
title_full STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT
title_fullStr STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT
title_full_unstemmed STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT
title_short STUDIES ON IMMUNE CELLULAR INJURY : I. CYTOTOXIC EFFECTS OF ANTIBODY AND COMPLEMENT
title_sort studies on immune cellular injury : i. cytotoxic effects of antibody and complement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137319/
https://www.ncbi.nlm.nih.gov/pubmed/13743575
work_keys_str_mv AT rossann studiesonimmunecellularinjuryicytotoxiceffectsofantibodyandcomplement
AT lepowirwinh studiesonimmunecellularinjuryicytotoxiceffectsofantibodyandcomplement

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