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PREPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION FROM HUMAN ERYTHROCYTES

A material, derived from human erythrocytes and believed to be identical with the receptor site for the myxoviruses, has been obtained in homogeneous form. The method of preparation involves pH adjustment of the stroma, hot phenolic extraction, chloroform-methanol treatment, and ultracentrifugation....

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Detalles Bibliográficos
Autores principales: Kathan, Ralph H., Winzler, Richard J., Johnson, Clarence A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137338/
https://www.ncbi.nlm.nih.gov/pubmed/13751754
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author Kathan, Ralph H.
Winzler, Richard J.
Johnson, Clarence A.
author_facet Kathan, Ralph H.
Winzler, Richard J.
Johnson, Clarence A.
author_sort Kathan, Ralph H.
collection PubMed
description A material, derived from human erythrocytes and believed to be identical with the receptor site for the myxoviruses, has been obtained in homogeneous form. The method of preparation involves pH adjustment of the stroma, hot phenolic extraction, chloroform-methanol treatment, and ultracentrifugation. The material so obtained possesses a high inhibitory titer to viral hemagglutination (45,000 to 60,000 inhibitory units/mg. against 4 hemagglutination units of Lee strain of influenza virus) and appears to be a glycoprotein containing 22 to 24 per cent sialic acid, 12 per cent hexose, 12 per cent hexosamine, 1 per cent fucose in addition to at least eleven amino acids. The sialic acid probably occupies a terminal position in an oligosaccharide chain extending from the protein peptide chain. The molecular weight is near 30,000—an unusually low value for substances possessing this biological activity. M and N blood group activity also seems to be associated with this protein.
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spelling pubmed-21373382008-04-17 PREPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION FROM HUMAN ERYTHROCYTES Kathan, Ralph H. Winzler, Richard J. Johnson, Clarence A. J Exp Med Article A material, derived from human erythrocytes and believed to be identical with the receptor site for the myxoviruses, has been obtained in homogeneous form. The method of preparation involves pH adjustment of the stroma, hot phenolic extraction, chloroform-methanol treatment, and ultracentrifugation. The material so obtained possesses a high inhibitory titer to viral hemagglutination (45,000 to 60,000 inhibitory units/mg. against 4 hemagglutination units of Lee strain of influenza virus) and appears to be a glycoprotein containing 22 to 24 per cent sialic acid, 12 per cent hexose, 12 per cent hexosamine, 1 per cent fucose in addition to at least eleven amino acids. The sialic acid probably occupies a terminal position in an oligosaccharide chain extending from the protein peptide chain. The molecular weight is near 30,000—an unusually low value for substances possessing this biological activity. M and N blood group activity also seems to be associated with this protein. The Rockefeller University Press 1961-01-01 /pmc/articles/PMC2137338/ /pubmed/13751754 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kathan, Ralph H.
Winzler, Richard J.
Johnson, Clarence A.
PREPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION FROM HUMAN ERYTHROCYTES
title PREPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION FROM HUMAN ERYTHROCYTES
title_full PREPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION FROM HUMAN ERYTHROCYTES
title_fullStr PREPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION FROM HUMAN ERYTHROCYTES
title_full_unstemmed PREPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION FROM HUMAN ERYTHROCYTES
title_short PREPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION FROM HUMAN ERYTHROCYTES
title_sort preparation of an inhibitor of viral hemagglutination from human erythrocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137338/
https://www.ncbi.nlm.nih.gov/pubmed/13751754
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