A SIMPLIFIED CHEMOSTAT FOR THE GROWTH OF MAMMALIAN CELLS: CHARACTERISTICS OF CELL GROWTH IN CONTINUOUS CULTURE

A simplified technique has been described for the continuous growth of mammalian cells in suspension culture. The cell population density increased as the rate of input of fresh medium was decreased, and the average generation time was concommittantly prolonged. At relatively high input rates, the p...

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Detalles Bibliográficos
Autores principales: Cohen, Edward P., Eagle, Harry
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137361/
https://www.ncbi.nlm.nih.gov/pubmed/13694368
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author Cohen, Edward P.
Eagle, Harry
author_facet Cohen, Edward P.
Eagle, Harry
author_sort Cohen, Edward P.
collection PubMed
description A simplified technique has been described for the continuous growth of mammalian cells in suspension culture. The cell population density increased as the rate of input of fresh medium was decreased, and the average generation time was concommittantly prolonged. At relatively high input rates, the population remained stabilized for an indefinite period, but at low flow rates, there was sometimes a cyclical variation in population density. The factor limiting growth rate at input rates of approximately 0.2 volumes per day was not the exhaustion of the medium; but in some experiments a non-dialyzable material appeared which inhibited cell growth.
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spelling pubmed-21373612008-04-17 A SIMPLIFIED CHEMOSTAT FOR THE GROWTH OF MAMMALIAN CELLS: CHARACTERISTICS OF CELL GROWTH IN CONTINUOUS CULTURE Cohen, Edward P. Eagle, Harry J Exp Med Article A simplified technique has been described for the continuous growth of mammalian cells in suspension culture. The cell population density increased as the rate of input of fresh medium was decreased, and the average generation time was concommittantly prolonged. At relatively high input rates, the population remained stabilized for an indefinite period, but at low flow rates, there was sometimes a cyclical variation in population density. The factor limiting growth rate at input rates of approximately 0.2 volumes per day was not the exhaustion of the medium; but in some experiments a non-dialyzable material appeared which inhibited cell growth. The Rockefeller University Press 1961-01-31 /pmc/articles/PMC2137361/ /pubmed/13694368 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Cohen, Edward P.
Eagle, Harry
A SIMPLIFIED CHEMOSTAT FOR THE GROWTH OF MAMMALIAN CELLS: CHARACTERISTICS OF CELL GROWTH IN CONTINUOUS CULTURE
title A SIMPLIFIED CHEMOSTAT FOR THE GROWTH OF MAMMALIAN CELLS: CHARACTERISTICS OF CELL GROWTH IN CONTINUOUS CULTURE
title_full A SIMPLIFIED CHEMOSTAT FOR THE GROWTH OF MAMMALIAN CELLS: CHARACTERISTICS OF CELL GROWTH IN CONTINUOUS CULTURE
title_fullStr A SIMPLIFIED CHEMOSTAT FOR THE GROWTH OF MAMMALIAN CELLS: CHARACTERISTICS OF CELL GROWTH IN CONTINUOUS CULTURE
title_full_unstemmed A SIMPLIFIED CHEMOSTAT FOR THE GROWTH OF MAMMALIAN CELLS: CHARACTERISTICS OF CELL GROWTH IN CONTINUOUS CULTURE
title_short A SIMPLIFIED CHEMOSTAT FOR THE GROWTH OF MAMMALIAN CELLS: CHARACTERISTICS OF CELL GROWTH IN CONTINUOUS CULTURE
title_sort simplified chemostat for the growth of mammalian cells: characteristics of cell growth in continuous culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137361/
https://www.ncbi.nlm.nih.gov/pubmed/13694368
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