THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS STAPHYLOCOCCAL PROTEIN POSSESSING ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL, AND LEUCOCIDAL ACTIVITIES

The continuous flow paper electrophoretic methods used to isolate alpha hemolysin are presented. The purity of the alpha hemolysin preparation is demonstrated by double agar diffusion, immunoelectrophoretic, and ultracentrifugation techniques. Indirect evidence of purity is provided by the fact that...

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Detalles Bibliográficos
Autores principales: Kumar, Surendra, Loken, Keith I., Kenyon, Alan J., Lindorfer, Robert K.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137398/
https://www.ncbi.nlm.nih.gov/pubmed/19867212
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author Kumar, Surendra
Loken, Keith I.
Kenyon, Alan J.
Lindorfer, Robert K.
author_facet Kumar, Surendra
Loken, Keith I.
Kenyon, Alan J.
Lindorfer, Robert K.
author_sort Kumar, Surendra
collection PubMed
description The continuous flow paper electrophoretic methods used to isolate alpha hemolysin are presented. The purity of the alpha hemolysin preparation is demonstrated by double agar diffusion, immunoelectrophoretic, and ultracentrifugation techniques. Indirect evidence of purity is provided by the fact that rabbit immunized with purified alpha hemolysin produced detectable antibody only to alpha hemolysin. Direct support is given to the "unitarian theory" by the demonstration that purified alpha hemolysin also possessed dermonecrotic, lethal, and leucocidal activities. Further studies dependent on the availability of purified alpha hemolysin are outlined.
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spelling pubmed-21373982008-04-17 THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS STAPHYLOCOCCAL PROTEIN POSSESSING ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL, AND LEUCOCIDAL ACTIVITIES Kumar, Surendra Loken, Keith I. Kenyon, Alan J. Lindorfer, Robert K. J Exp Med Article The continuous flow paper electrophoretic methods used to isolate alpha hemolysin are presented. The purity of the alpha hemolysin preparation is demonstrated by double agar diffusion, immunoelectrophoretic, and ultracentrifugation techniques. Indirect evidence of purity is provided by the fact that rabbit immunized with purified alpha hemolysin produced detectable antibody only to alpha hemolysin. Direct support is given to the "unitarian theory" by the demonstration that purified alpha hemolysin also possessed dermonecrotic, lethal, and leucocidal activities. Further studies dependent on the availability of purified alpha hemolysin are outlined. The Rockefeller University Press 1962-06-01 /pmc/articles/PMC2137398/ /pubmed/19867212 Text en Copyright © Copyright, 1962, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kumar, Surendra
Loken, Keith I.
Kenyon, Alan J.
Lindorfer, Robert K.
THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS STAPHYLOCOCCAL PROTEIN POSSESSING ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL, AND LEUCOCIDAL ACTIVITIES
title THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS STAPHYLOCOCCAL PROTEIN POSSESSING ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL, AND LEUCOCIDAL ACTIVITIES
title_full THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS STAPHYLOCOCCAL PROTEIN POSSESSING ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL, AND LEUCOCIDAL ACTIVITIES
title_fullStr THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS STAPHYLOCOCCAL PROTEIN POSSESSING ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL, AND LEUCOCIDAL ACTIVITIES
title_full_unstemmed THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS STAPHYLOCOCCAL PROTEIN POSSESSING ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL, AND LEUCOCIDAL ACTIVITIES
title_short THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS STAPHYLOCOCCAL PROTEIN POSSESSING ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL, AND LEUCOCIDAL ACTIVITIES
title_sort characterization of staphylococcal toxins : ii. the isolation and characterization of a homogeneous staphylococcal protein possessing alpha hemolytic, dermonecrotic, lethal, and leucocidal activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137398/
https://www.ncbi.nlm.nih.gov/pubmed/19867212
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