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THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : I. THE ELECTROPHORETIC MIGRATION OF THE ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL AND LEUCOCIDAL ACTIVITIES OF CRUDE TOXIN
The continuous flow paper electrophoretic methods used to isolate alpha hemolysin are presented. The purity of the alpha hemolysin preparation is demonstrated by double agar diffusion, immunoelectrophoretic, and ultracentrifugation techniques. Indirect evidence of purity is provided by the fact that...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1962
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137403/ https://www.ncbi.nlm.nih.gov/pubmed/14460502 |
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author | Kumar, Surendra Lindorfer, Robert K. |
author_facet | Kumar, Surendra Lindorfer, Robert K. |
author_sort | Kumar, Surendra |
collection | PubMed |
description | The continuous flow paper electrophoretic methods used to isolate alpha hemolysin are presented. The purity of the alpha hemolysin preparation is demonstrated by double agar diffusion, immunoelectrophoretic, and ultracentrifugation techniques. Indirect evidence of purity is provided by the fact that rabbits immunized with purified alpha hemolysin produced detectable antibody only to alpha hemolysin. Direct support is given to the "unitarian theory" by the demonstration that purified alpha hemolysin also possessed dermonecrotic, lethal, and leucocidal activities. Further studies dependent on the availability of purified alpha hemolysin are outlined. |
format | Text |
id | pubmed-2137403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1962 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21374032008-04-17 THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : I. THE ELECTROPHORETIC MIGRATION OF THE ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL AND LEUCOCIDAL ACTIVITIES OF CRUDE TOXIN Kumar, Surendra Lindorfer, Robert K. J Exp Med Article The continuous flow paper electrophoretic methods used to isolate alpha hemolysin are presented. The purity of the alpha hemolysin preparation is demonstrated by double agar diffusion, immunoelectrophoretic, and ultracentrifugation techniques. Indirect evidence of purity is provided by the fact that rabbits immunized with purified alpha hemolysin produced detectable antibody only to alpha hemolysin. Direct support is given to the "unitarian theory" by the demonstration that purified alpha hemolysin also possessed dermonecrotic, lethal, and leucocidal activities. Further studies dependent on the availability of purified alpha hemolysin are outlined. The Rockefeller University Press 1962-06-01 /pmc/articles/PMC2137403/ /pubmed/14460502 Text en Copyright © Copyright, 1962, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Kumar, Surendra Lindorfer, Robert K. THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : I. THE ELECTROPHORETIC MIGRATION OF THE ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL AND LEUCOCIDAL ACTIVITIES OF CRUDE TOXIN |
title | THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : I. THE ELECTROPHORETIC MIGRATION OF THE ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL AND LEUCOCIDAL ACTIVITIES OF CRUDE TOXIN |
title_full | THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : I. THE ELECTROPHORETIC MIGRATION OF THE ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL AND LEUCOCIDAL ACTIVITIES OF CRUDE TOXIN |
title_fullStr | THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : I. THE ELECTROPHORETIC MIGRATION OF THE ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL AND LEUCOCIDAL ACTIVITIES OF CRUDE TOXIN |
title_full_unstemmed | THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : I. THE ELECTROPHORETIC MIGRATION OF THE ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL AND LEUCOCIDAL ACTIVITIES OF CRUDE TOXIN |
title_short | THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : I. THE ELECTROPHORETIC MIGRATION OF THE ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL AND LEUCOCIDAL ACTIVITIES OF CRUDE TOXIN |
title_sort | characterization of staphylococcal toxins : i. the electrophoretic migration of the alpha hemolytic, dermonecrotic, lethal and leucocidal activities of crude toxin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137403/ https://www.ncbi.nlm.nih.gov/pubmed/14460502 |
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