STUDIES ON CELL LINES DEVELOPED FROM THE TISSUES OF PATIENTS WITH GALACTOSEMIA

Cell lines were developed from biopsies on galactosemic and non-galactosemic patients. It was shown that one can discriminate between lines from the two types of donors by their relative growth in glucose and galactose and by their ability to oxidize galactose-1-C(14). The latter method was successf...

Descripción completa

Detalles Bibliográficos
Autores principales: Krooth, Robert S., Weinberg, Arnold N.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137427/
https://www.ncbi.nlm.nih.gov/pubmed/13754521
_version_ 1782143329176125440
author Krooth, Robert S.
Weinberg, Arnold N.
author_facet Krooth, Robert S.
Weinberg, Arnold N.
author_sort Krooth, Robert S.
collection PubMed
description Cell lines were developed from biopsies on galactosemic and non-galactosemic patients. It was shown that one can discriminate between lines from the two types of donors by their relative growth in glucose and galactose and by their ability to oxidize galactose-1-C(14). The latter method was successful in distinguishing a heterozygous cell line from the normal ones. Sensitivity of galactosemic cells to galactose was suggested by some of the experiments. The kinetics of growth were in some ways reminiscent of a similar phenomenon in the transferase mutants of E. coli, though in the human cells the effect was much less marked.
format Text
id pubmed-2137427
institution National Center for Biotechnology Information
language English
publishDate 1961
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21374272008-04-17 STUDIES ON CELL LINES DEVELOPED FROM THE TISSUES OF PATIENTS WITH GALACTOSEMIA Krooth, Robert S. Weinberg, Arnold N. J Exp Med Article Cell lines were developed from biopsies on galactosemic and non-galactosemic patients. It was shown that one can discriminate between lines from the two types of donors by their relative growth in glucose and galactose and by their ability to oxidize galactose-1-C(14). The latter method was successful in distinguishing a heterozygous cell line from the normal ones. Sensitivity of galactosemic cells to galactose was suggested by some of the experiments. The kinetics of growth were in some ways reminiscent of a similar phenomenon in the transferase mutants of E. coli, though in the human cells the effect was much less marked. The Rockefeller University Press 1961-06-01 /pmc/articles/PMC2137427/ /pubmed/13754521 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Krooth, Robert S.
Weinberg, Arnold N.
STUDIES ON CELL LINES DEVELOPED FROM THE TISSUES OF PATIENTS WITH GALACTOSEMIA
title STUDIES ON CELL LINES DEVELOPED FROM THE TISSUES OF PATIENTS WITH GALACTOSEMIA
title_full STUDIES ON CELL LINES DEVELOPED FROM THE TISSUES OF PATIENTS WITH GALACTOSEMIA
title_fullStr STUDIES ON CELL LINES DEVELOPED FROM THE TISSUES OF PATIENTS WITH GALACTOSEMIA
title_full_unstemmed STUDIES ON CELL LINES DEVELOPED FROM THE TISSUES OF PATIENTS WITH GALACTOSEMIA
title_short STUDIES ON CELL LINES DEVELOPED FROM THE TISSUES OF PATIENTS WITH GALACTOSEMIA
title_sort studies on cell lines developed from the tissues of patients with galactosemia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137427/
https://www.ncbi.nlm.nih.gov/pubmed/13754521
work_keys_str_mv AT kroothroberts studiesoncelllinesdevelopedfromthetissuesofpatientswithgalactosemia
AT weinbergarnoldn studiesoncelllinesdevelopedfromthetissuesofpatientswithgalactosemia

Ejemplares similares