AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES

A new character, the A marker, for polioviruses is described. In the presence of Al(+++) (1 to 100 mM) attenuated, but not virulent, strains of type 1 and type 2 polioviruses are stabilized so that they resist thermal inactivation at 50°C. Differences between the attenuated and virulent type 3 strai...

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Detalles Bibliográficos
Autores principales: Wallis, Craig, Melnick, Joseph L., Ferry, George D., Wimberly, Ira L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137513/
https://www.ncbi.nlm.nih.gov/pubmed/14004705
Descripción
Sumario:A new character, the A marker, for polioviruses is described. In the presence of Al(+++) (1 to 100 mM) attenuated, but not virulent, strains of type 1 and type 2 polioviruses are stabilized so that they resist thermal inactivation at 50°C. Differences between the attenuated and virulent type 3 strains studied were of the opposite character and less marked. By the use of optimal concentrations of Al(+++) (12 mM for type 1 and 1 mM for type 2) attenuated strains can be separated from mixtures containing virulent type 1 or 2 strains. The A marker was found to be correlated with the d but not the rct/40 or T marker. The A marker has been used for following genetic changes of vaccine strains after their multiplication in man.

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