AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES

A new character, the A marker, for polioviruses is described. In the presence of Al(+++) (1 to 100 mM) attenuated, but not virulent, strains of type 1 and type 2 polioviruses are stabilized so that they resist thermal inactivation at 50°C. Differences between the attenuated and virulent type 3 strai...

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Detalles Bibliográficos
Autores principales: Wallis, Craig, Melnick, Joseph L., Ferry, George D., Wimberly, Ira L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137513/
https://www.ncbi.nlm.nih.gov/pubmed/14004705
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author Wallis, Craig
Melnick, Joseph L.
Ferry, George D.
Wimberly, Ira L.
author_facet Wallis, Craig
Melnick, Joseph L.
Ferry, George D.
Wimberly, Ira L.
author_sort Wallis, Craig
collection PubMed
description A new character, the A marker, for polioviruses is described. In the presence of Al(+++) (1 to 100 mM) attenuated, but not virulent, strains of type 1 and type 2 polioviruses are stabilized so that they resist thermal inactivation at 50°C. Differences between the attenuated and virulent type 3 strains studied were of the opposite character and less marked. By the use of optimal concentrations of Al(+++) (12 mM for type 1 and 1 mM for type 2) attenuated strains can be separated from mixtures containing virulent type 1 or 2 strains. The A marker was found to be correlated with the d but not the rct/40 or T marker. The A marker has been used for following genetic changes of vaccine strains after their multiplication in man.
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spelling pubmed-21375132008-04-17 AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES Wallis, Craig Melnick, Joseph L. Ferry, George D. Wimberly, Ira L. J Exp Med Article A new character, the A marker, for polioviruses is described. In the presence of Al(+++) (1 to 100 mM) attenuated, but not virulent, strains of type 1 and type 2 polioviruses are stabilized so that they resist thermal inactivation at 50°C. Differences between the attenuated and virulent type 3 strains studied were of the opposite character and less marked. By the use of optimal concentrations of Al(+++) (12 mM for type 1 and 1 mM for type 2) attenuated strains can be separated from mixtures containing virulent type 1 or 2 strains. The A marker was found to be correlated with the d but not the rct/40 or T marker. The A marker has been used for following genetic changes of vaccine strains after their multiplication in man. The Rockefeller University Press 1962-03-31 /pmc/articles/PMC2137513/ /pubmed/14004705 Text en Copyright © Copyright, 1962, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wallis, Craig
Melnick, Joseph L.
Ferry, George D.
Wimberly, Ira L.
AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES
title AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES
title_full AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES
title_fullStr AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES
title_full_unstemmed AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES
title_short AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES
title_sort aluminum marker for the differentiation and separation of virulent and attenuated polioviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137513/
https://www.ncbi.nlm.nih.gov/pubmed/14004705
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