EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS : I. PROPERTIES OF THE L-ASPARAGINASE OF GUINEA PIG SERUM IN RELATION TO THOSE OF THE ANTILYMPHOMA SUBSTANCE

A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found...

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Detalles Bibliográficos
Autor principal: Broome, J. D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137570/
https://www.ncbi.nlm.nih.gov/pubmed/14015821
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author Broome, J. D.
author_facet Broome, J. D.
author_sort Broome, J. D.
collection PubMed
description A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found to parallel closely those of the antilymphoma agent. L-Asparaginase activity was essentially absent from the serum of newborn guinea pigs and this failed to inhibit 6C3HED cells. On separating guinea pig serum proteins by salt precipitation, electrophoresis, and chromatography on DEAE cellulose, antilymphoma activity was found only in fractions which contained L-asparaginase.
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spelling pubmed-21375702008-04-17 EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS : I. PROPERTIES OF THE L-ASPARAGINASE OF GUINEA PIG SERUM IN RELATION TO THOSE OF THE ANTILYMPHOMA SUBSTANCE Broome, J. D. J Exp Med Article A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found to parallel closely those of the antilymphoma agent. L-Asparaginase activity was essentially absent from the serum of newborn guinea pigs and this failed to inhibit 6C3HED cells. On separating guinea pig serum proteins by salt precipitation, electrophoresis, and chromatography on DEAE cellulose, antilymphoma activity was found only in fractions which contained L-asparaginase. The Rockefeller University Press 1963-07-01 /pmc/articles/PMC2137570/ /pubmed/14015821 Text en Copyright © 1963, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Broome, J. D.
EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS : I. PROPERTIES OF THE L-ASPARAGINASE OF GUINEA PIG SERUM IN RELATION TO THOSE OF THE ANTILYMPHOMA SUBSTANCE
title EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS : I. PROPERTIES OF THE L-ASPARAGINASE OF GUINEA PIG SERUM IN RELATION TO THOSE OF THE ANTILYMPHOMA SUBSTANCE
title_full EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS : I. PROPERTIES OF THE L-ASPARAGINASE OF GUINEA PIG SERUM IN RELATION TO THOSE OF THE ANTILYMPHOMA SUBSTANCE
title_fullStr EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS : I. PROPERTIES OF THE L-ASPARAGINASE OF GUINEA PIG SERUM IN RELATION TO THOSE OF THE ANTILYMPHOMA SUBSTANCE
title_full_unstemmed EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS : I. PROPERTIES OF THE L-ASPARAGINASE OF GUINEA PIG SERUM IN RELATION TO THOSE OF THE ANTILYMPHOMA SUBSTANCE
title_short EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS : I. PROPERTIES OF THE L-ASPARAGINASE OF GUINEA PIG SERUM IN RELATION TO THOSE OF THE ANTILYMPHOMA SUBSTANCE
title_sort evidence that the l-asparaginase of guinea pig serum is responsible for its antilymphoma effects : i. properties of the l-asparaginase of guinea pig serum in relation to those of the antilymphoma substance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137570/
https://www.ncbi.nlm.nih.gov/pubmed/14015821
work_keys_str_mv AT broomejd evidencethatthelasparaginaseofguineapigserumisresponsibleforitsantilymphomaeffectsipropertiesofthelasparaginaseofguineapigseruminrelationtothoseoftheantilymphomasubstance

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