Cargando…
EXPERIMENTAL LATHYRISM IN THE CHICK EMBRYO : THE DISTRIBUTION OF β-AMINOPROPIONITRILE
1. C(14)-labeled β-aminopropionitrile distributed throughout the egg contents within 10 minutes postinjection. By ion exchange chromatography and electrophoretic analysis three major components of the extractable dialyzable radioactive material could be demonstrated, representing at least 80 per cen...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1963
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137590/ https://www.ncbi.nlm.nih.gov/pubmed/13940344 |
_version_ | 1782143367542472704 |
---|---|
author | Orloff, Serge D. Gross, Jerome |
author_facet | Orloff, Serge D. Gross, Jerome |
author_sort | Orloff, Serge D. |
collection | PubMed |
description | 1. C(14)-labeled β-aminopropionitrile distributed throughout the egg contents within 10 minutes postinjection. By ion exchange chromatography and electrophoretic analysis three major components of the extractable dialyzable radioactive material could be demonstrated, representing at least 80 per cent of the total. The acidic and basic components were identified as β-aminopropionitrile and cyanoacetic acid, while the fraction isoelectric at pH 5.3, consisting of two components, remained unidentified. 2. Less than 1 molecule of βAPN per 100 molecules of protein was present in the highly purified extractable lathyritic bone collagen indicating that binding of the lathyrogen is not a factor in collagen extractability. 3. The proximity of βAPN to collagen in bone is not essential to its extractability. 4. The effect of incubation temperature of the embryo on collagen extractability suggests the involvement of a metabolic process in this phenomenon. |
format | Text |
id | pubmed-2137590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1963 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21375902008-04-17 EXPERIMENTAL LATHYRISM IN THE CHICK EMBRYO : THE DISTRIBUTION OF β-AMINOPROPIONITRILE Orloff, Serge D. Gross, Jerome J Exp Med Article 1. C(14)-labeled β-aminopropionitrile distributed throughout the egg contents within 10 minutes postinjection. By ion exchange chromatography and electrophoretic analysis three major components of the extractable dialyzable radioactive material could be demonstrated, representing at least 80 per cent of the total. The acidic and basic components were identified as β-aminopropionitrile and cyanoacetic acid, while the fraction isoelectric at pH 5.3, consisting of two components, remained unidentified. 2. Less than 1 molecule of βAPN per 100 molecules of protein was present in the highly purified extractable lathyritic bone collagen indicating that binding of the lathyrogen is not a factor in collagen extractability. 3. The proximity of βAPN to collagen in bone is not essential to its extractability. 4. The effect of incubation temperature of the embryo on collagen extractability suggests the involvement of a metabolic process in this phenomenon. The Rockefeller University Press 1963-06-01 /pmc/articles/PMC2137590/ /pubmed/13940344 Text en Copyright ©, 1963, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Orloff, Serge D. Gross, Jerome EXPERIMENTAL LATHYRISM IN THE CHICK EMBRYO : THE DISTRIBUTION OF β-AMINOPROPIONITRILE |
title | EXPERIMENTAL LATHYRISM IN THE CHICK EMBRYO : THE DISTRIBUTION OF β-AMINOPROPIONITRILE |
title_full | EXPERIMENTAL LATHYRISM IN THE CHICK EMBRYO : THE DISTRIBUTION OF β-AMINOPROPIONITRILE |
title_fullStr | EXPERIMENTAL LATHYRISM IN THE CHICK EMBRYO : THE DISTRIBUTION OF β-AMINOPROPIONITRILE |
title_full_unstemmed | EXPERIMENTAL LATHYRISM IN THE CHICK EMBRYO : THE DISTRIBUTION OF β-AMINOPROPIONITRILE |
title_short | EXPERIMENTAL LATHYRISM IN THE CHICK EMBRYO : THE DISTRIBUTION OF β-AMINOPROPIONITRILE |
title_sort | experimental lathyrism in the chick embryo : the distribution of β-aminopropionitrile |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137590/ https://www.ncbi.nlm.nih.gov/pubmed/13940344 |
work_keys_str_mv | AT orloffserged experimentallathyrisminthechickembryothedistributionofbaminopropionitrile AT grossjerome experimentallathyrisminthechickembryothedistributionofbaminopropionitrile |