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ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN

The diffusion chamber technique permitted the demonstration of specific antibody formation in x-irradiated recipients of such chambers filled with normal lymph node cells and a cell-free homogenate of macrophages which had been incubated in intro with T2 bacteriophage. The activity of the cell-free...

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Detalles Bibliográficos
Autores principales: Fishman, M., Adler, F. L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137621/
https://www.ncbi.nlm.nih.gov/pubmed/13945311
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author Fishman, M.
Adler, F. L.
author_facet Fishman, M.
Adler, F. L.
author_sort Fishman, M.
collection PubMed
description The diffusion chamber technique permitted the demonstration of specific antibody formation in x-irradiated recipients of such chambers filled with normal lymph node cells and a cell-free homogenate of macrophages which had been incubated in intro with T2 bacteriophage. The activity of the cell-free homogenate was retained in its RNA fraction isolated by means of the phenol method. No antibody formation occurred if such RNA was treated with RNAase. On sucrose gradients (5 to 20 per cent), the active RNA was found to be present in the top third layer. The question of the possible presence of antigen complexed to the RNA is discussed.
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spelling pubmed-21376212008-04-17 ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN Fishman, M. Adler, F. L. J Exp Med Article The diffusion chamber technique permitted the demonstration of specific antibody formation in x-irradiated recipients of such chambers filled with normal lymph node cells and a cell-free homogenate of macrophages which had been incubated in intro with T2 bacteriophage. The activity of the cell-free homogenate was retained in its RNA fraction isolated by means of the phenol method. No antibody formation occurred if such RNA was treated with RNAase. On sucrose gradients (5 to 20 per cent), the active RNA was found to be present in the top third layer. The question of the possible presence of antigen complexed to the RNA is discussed. The Rockefeller University Press 1963-03-31 /pmc/articles/PMC2137621/ /pubmed/13945311 Text en Copyright ©, 1963, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Fishman, M.
Adler, F. L.
ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN
title ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN
title_full ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN
title_fullStr ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN
title_full_unstemmed ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN
title_short ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN
title_sort antibody formation initiated in vitro : ii. antibody synthesis in x-irradiated recipients of diffusion chambers containing nucleic acid derived from macrophages incubated with antigen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137621/
https://www.ncbi.nlm.nih.gov/pubmed/13945311
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AT adlerfl antibodyformationinitiatedinvitroiiantibodysynthesisinxirradiatedrecipientsofdiffusionchamberscontainingnucleicacidderivedfrommacrophagesincubatedwithantigen