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ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN
The diffusion chamber technique permitted the demonstration of specific antibody formation in x-irradiated recipients of such chambers filled with normal lymph node cells and a cell-free homogenate of macrophages which had been incubated in intro with T2 bacteriophage. The activity of the cell-free...
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1963
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137621/ https://www.ncbi.nlm.nih.gov/pubmed/13945311 |
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author | Fishman, M. Adler, F. L. |
author_facet | Fishman, M. Adler, F. L. |
author_sort | Fishman, M. |
collection | PubMed |
description | The diffusion chamber technique permitted the demonstration of specific antibody formation in x-irradiated recipients of such chambers filled with normal lymph node cells and a cell-free homogenate of macrophages which had been incubated in intro with T2 bacteriophage. The activity of the cell-free homogenate was retained in its RNA fraction isolated by means of the phenol method. No antibody formation occurred if such RNA was treated with RNAase. On sucrose gradients (5 to 20 per cent), the active RNA was found to be present in the top third layer. The question of the possible presence of antigen complexed to the RNA is discussed. |
format | Text |
id | pubmed-2137621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1963 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21376212008-04-17 ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN Fishman, M. Adler, F. L. J Exp Med Article The diffusion chamber technique permitted the demonstration of specific antibody formation in x-irradiated recipients of such chambers filled with normal lymph node cells and a cell-free homogenate of macrophages which had been incubated in intro with T2 bacteriophage. The activity of the cell-free homogenate was retained in its RNA fraction isolated by means of the phenol method. No antibody formation occurred if such RNA was treated with RNAase. On sucrose gradients (5 to 20 per cent), the active RNA was found to be present in the top third layer. The question of the possible presence of antigen complexed to the RNA is discussed. The Rockefeller University Press 1963-03-31 /pmc/articles/PMC2137621/ /pubmed/13945311 Text en Copyright ©, 1963, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Fishman, M. Adler, F. L. ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN |
title | ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN |
title_full | ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN |
title_fullStr | ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN |
title_full_unstemmed | ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN |
title_short | ANTIBODY FORMATION INITIATED IN VITRO : II. ANTIBODY SYNTHESIS IN X-IRRADIATED RECIPIENTS OF DIFFUSION CHAMBERS CONTAINING NUCLEIC ACID DERIVED FROM MACROPHAGES INCUBATED WITH ANTIGEN |
title_sort | antibody formation initiated in vitro : ii. antibody synthesis in x-irradiated recipients of diffusion chambers containing nucleic acid derived from macrophages incubated with antigen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137621/ https://www.ncbi.nlm.nih.gov/pubmed/13945311 |
work_keys_str_mv | AT fishmanm antibodyformationinitiatedinvitroiiantibodysynthesisinxirradiatedrecipientsofdiffusionchamberscontainingnucleicacidderivedfrommacrophagesincubatedwithantigen AT adlerfl antibodyformationinitiatedinvitroiiantibodysynthesisinxirradiatedrecipientsofdiffusionchamberscontainingnucleicacidderivedfrommacrophagesincubatedwithantigen |