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ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT
Purification of the activity of the fourth component of human complement resulted in the isolation of a highly homogeneous serum protein. Since this protein has not been recorded previously it was called β(1E)-globulin on the basis of its immunoelectrophoretic behavior. C'4 activity and β(1E)-g...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1963
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137649/ https://www.ncbi.nlm.nih.gov/pubmed/14078003 |
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author | Müller-Eberhard, H. J. Biro, C. E. |
author_facet | Müller-Eberhard, H. J. Biro, C. E. |
author_sort | Müller-Eberhard, H. J. |
collection | PubMed |
description | Purification of the activity of the fourth component of human complement resulted in the isolation of a highly homogeneous serum protein. Since this protein has not been recorded previously it was called β(1E)-globulin on the basis of its immunoelectrophoretic behavior. C'4 activity and β(1E)-globulin were found to have highly similar, if not identical physicochemical characteristics. Moreover, β(1E)-globulin was shown to exhibit the specific behavior of C'4 activity in that it is taken up only by cells which contain activated C'1. DFP-inactivated C'1 failed to catalyze uptake of the protein. Treatment with hydrazine which is known to destroy C'4 activity, led to changes in the physicochemical properties of β(1E)-globulin and rendered the molecule incapable to combine with C'1-containing cells. The evidence indicates that β(1E)-globulin represents the fourth component of human complement. |
format | Text |
id | pubmed-2137649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1963 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21376492008-04-17 ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT Müller-Eberhard, H. J. Biro, C. E. J Exp Med Article Purification of the activity of the fourth component of human complement resulted in the isolation of a highly homogeneous serum protein. Since this protein has not been recorded previously it was called β(1E)-globulin on the basis of its immunoelectrophoretic behavior. C'4 activity and β(1E)-globulin were found to have highly similar, if not identical physicochemical characteristics. Moreover, β(1E)-globulin was shown to exhibit the specific behavior of C'4 activity in that it is taken up only by cells which contain activated C'1. DFP-inactivated C'1 failed to catalyze uptake of the protein. Treatment with hydrazine which is known to destroy C'4 activity, led to changes in the physicochemical properties of β(1E)-globulin and rendered the molecule incapable to combine with C'1-containing cells. The evidence indicates that β(1E)-globulin represents the fourth component of human complement. The Rockefeller University Press 1963-09-01 /pmc/articles/PMC2137649/ /pubmed/14078003 Text en Copyright © 1963, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Müller-Eberhard, H. J. Biro, C. E. ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT |
title | ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT |
title_full | ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT |
title_fullStr | ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT |
title_full_unstemmed | ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT |
title_short | ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT |
title_sort | isolation and description of the fourth component of human complement |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137649/ https://www.ncbi.nlm.nih.gov/pubmed/14078003 |
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