RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA

The rheumatoid factor-like substance (RFLS) induced by hyperimmunization of rabbits with bacteria was consistently and exclusively associated with the macroglobulin fraction of the sera studied. The RFLS is separable from the bulk of serum proteins by zone centrifugation, DEAE cellulose chromatograp...

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Detalles Bibliográficos
Autor principal: Christian, Charles L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137676/
https://www.ncbi.nlm.nih.gov/pubmed/14087624
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author Christian, Charles L.
author_facet Christian, Charles L.
author_sort Christian, Charles L.
collection PubMed
description The rheumatoid factor-like substance (RFLS) induced by hyperimmunization of rabbits with bacteria was consistently and exclusively associated with the macroglobulin fraction of the sera studied. The RFLS is separable from the bulk of serum proteins by zone centrifugation, DEAE cellulose chromatography, and gel filtration. Partially purified preparations of the RFLS were studied by immunoelectrophoretic analysis and analytical ultracentrifugation. Certain RFLS sera demonstrated selective reactivity with one or more rabbit isohemagglutinin antibodies. Pepsin treatment of rabbit γ-globulin removed or greatly diminishes its capacity to react with the RFLS.
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spelling pubmed-21376762008-04-17 RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA Christian, Charles L. J Exp Med Article The rheumatoid factor-like substance (RFLS) induced by hyperimmunization of rabbits with bacteria was consistently and exclusively associated with the macroglobulin fraction of the sera studied. The RFLS is separable from the bulk of serum proteins by zone centrifugation, DEAE cellulose chromatography, and gel filtration. Partially purified preparations of the RFLS were studied by immunoelectrophoretic analysis and analytical ultracentrifugation. Certain RFLS sera demonstrated selective reactivity with one or more rabbit isohemagglutinin antibodies. Pepsin treatment of rabbit γ-globulin removed or greatly diminishes its capacity to react with the RFLS. The Rockefeller University Press 1963-10-31 /pmc/articles/PMC2137676/ /pubmed/14087624 Text en Copyright © 1963, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Christian, Charles L.
RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA
title RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA
title_full RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA
title_fullStr RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA
title_full_unstemmed RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA
title_short RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA
title_sort rheumatoid factor properties of hyperimmune rabbit sera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137676/
https://www.ncbi.nlm.nih.gov/pubmed/14087624
work_keys_str_mv AT christiancharlesl rheumatoidfactorpropertiesofhyperimmunerabbitsera

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