A STUDY OF THE DISTRIBUTION OF INCOMPLETE RHESUS ANTIBODIES AMONG THE SERUM IMMUNOGLOBULIN FRACTIONS

Sera containing Rh antibodies were taken from Rh-negative women at intervals during pregnancy and postpartum. A selection of these was fractionated on DEAE cellulose and by density gradient ultracentrifugation. The distribution among the immunoglobulin fractions γ(SS), γ(1)A, and γ(1)M of Rh antibod...

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Detalles Bibliográficos
Autores principales: Dodd, Barbara E., Wilkinson, P. C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1964
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137719/
https://www.ncbi.nlm.nih.gov/pubmed/14194392
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author Dodd, Barbara E.
Wilkinson, P. C.
author_facet Dodd, Barbara E.
Wilkinson, P. C.
author_sort Dodd, Barbara E.
collection PubMed
description Sera containing Rh antibodies were taken from Rh-negative women at intervals during pregnancy and postpartum. A selection of these was fractionated on DEAE cellulose and by density gradient ultracentrifugation. The distribution among the immunoglobulin fractions γ(SS), γ(1)A, and γ(1)M of Rh antibodies in the sera of Rh-negative women during pregnancy was analysed by these fractionation procedures and also by treatment with 2-mercaptoethanol and by testing with rabbit antisera prepared against purified immunoglobulin fractions. The experiments showed that incomplete Rh antibodies occurred in the 7(SS)-, γ(1)A-, and γ(1)M-globulin fractions, although the bulk of the antibody was in the 7(SS). In addition, evidence that the previously described (13) "papain-type" antibody is an incomplete γ(1)M-globulin is presented.
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spelling pubmed-21377192008-04-17 A STUDY OF THE DISTRIBUTION OF INCOMPLETE RHESUS ANTIBODIES AMONG THE SERUM IMMUNOGLOBULIN FRACTIONS Dodd, Barbara E. Wilkinson, P. C. J Exp Med Article Sera containing Rh antibodies were taken from Rh-negative women at intervals during pregnancy and postpartum. A selection of these was fractionated on DEAE cellulose and by density gradient ultracentrifugation. The distribution among the immunoglobulin fractions γ(SS), γ(1)A, and γ(1)M of Rh antibodies in the sera of Rh-negative women during pregnancy was analysed by these fractionation procedures and also by treatment with 2-mercaptoethanol and by testing with rabbit antisera prepared against purified immunoglobulin fractions. The experiments showed that incomplete Rh antibodies occurred in the 7(SS)-, γ(1)A-, and γ(1)M-globulin fractions, although the bulk of the antibody was in the 7(SS). In addition, evidence that the previously described (13) "papain-type" antibody is an incomplete γ(1)M-globulin is presented. The Rockefeller University Press 1964-07-01 /pmc/articles/PMC2137719/ /pubmed/14194392 Text en Copyright © 1964 by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Dodd, Barbara E.
Wilkinson, P. C.
A STUDY OF THE DISTRIBUTION OF INCOMPLETE RHESUS ANTIBODIES AMONG THE SERUM IMMUNOGLOBULIN FRACTIONS
title A STUDY OF THE DISTRIBUTION OF INCOMPLETE RHESUS ANTIBODIES AMONG THE SERUM IMMUNOGLOBULIN FRACTIONS
title_full A STUDY OF THE DISTRIBUTION OF INCOMPLETE RHESUS ANTIBODIES AMONG THE SERUM IMMUNOGLOBULIN FRACTIONS
title_fullStr A STUDY OF THE DISTRIBUTION OF INCOMPLETE RHESUS ANTIBODIES AMONG THE SERUM IMMUNOGLOBULIN FRACTIONS
title_full_unstemmed A STUDY OF THE DISTRIBUTION OF INCOMPLETE RHESUS ANTIBODIES AMONG THE SERUM IMMUNOGLOBULIN FRACTIONS
title_short A STUDY OF THE DISTRIBUTION OF INCOMPLETE RHESUS ANTIBODIES AMONG THE SERUM IMMUNOGLOBULIN FRACTIONS
title_sort study of the distribution of incomplete rhesus antibodies among the serum immunoglobulin fractions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137719/
https://www.ncbi.nlm.nih.gov/pubmed/14194392
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