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STUDIES ON THE PATHOGENESIS OF FEVER : XII. ELECTROLYTIC FACTORS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUCOCYTES
The metabolic reactions responsible for the release of endogenous pyrogen from rabbit granulocytes incubated in 0.15 M NaCl are specifically inhibited by the presence of K(+) (and by related alkali metal ions, Rb(+) and Cs(+)) in the medium. The inhibitory action of K(+) apparently involves penetrat...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1964
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137745/ https://www.ncbi.nlm.nih.gov/pubmed/14159044 |
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author | Berlin, Richard D. Wood, W. Barry |
author_facet | Berlin, Richard D. Wood, W. Barry |
author_sort | Berlin, Richard D. |
collection | PubMed |
description | The metabolic reactions responsible for the release of endogenous pyrogen from rabbit granulocytes incubated in 0.15 M NaCl are specifically inhibited by the presence of K(+) (and by related alkali metal ions, Rb(+) and Cs(+)) in the medium. The inhibitory action of K(+) apparently involves penetration of the cell membrane and is directly antagonized by the cardiac glycoside, ouabain. It is concluded, therefore, that the inhibition of pyrogen release by extracellular K(+) is due to transport of K(+) into the cell. Although the precise molecular mechanisms which are responsible for the release of pyrogen from granulocytes incubated in K-free saline have not been elucidated, further study of the process has revealed: (a) that it is preceded by the accumulation of pyrogen within the cell, (b) that it depends upon the catalytic action of one or more sulfhydryl-containing enzymes, (c) that it does not require energy, either from glycolysis or from reactions depending on molecular oxygen, and (d) that its inhibition by K(+) and by arsenite is qualitatively similar to the depression caused by these same reagents on the release of other leucocytic proteins; i.e., lysozyme and aldolase. |
format | Text |
id | pubmed-2137745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1964 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21377452008-04-17 STUDIES ON THE PATHOGENESIS OF FEVER : XII. ELECTROLYTIC FACTORS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUCOCYTES Berlin, Richard D. Wood, W. Barry J Exp Med Article The metabolic reactions responsible for the release of endogenous pyrogen from rabbit granulocytes incubated in 0.15 M NaCl are specifically inhibited by the presence of K(+) (and by related alkali metal ions, Rb(+) and Cs(+)) in the medium. The inhibitory action of K(+) apparently involves penetration of the cell membrane and is directly antagonized by the cardiac glycoside, ouabain. It is concluded, therefore, that the inhibition of pyrogen release by extracellular K(+) is due to transport of K(+) into the cell. Although the precise molecular mechanisms which are responsible for the release of pyrogen from granulocytes incubated in K-free saline have not been elucidated, further study of the process has revealed: (a) that it is preceded by the accumulation of pyrogen within the cell, (b) that it depends upon the catalytic action of one or more sulfhydryl-containing enzymes, (c) that it does not require energy, either from glycolysis or from reactions depending on molecular oxygen, and (d) that its inhibition by K(+) and by arsenite is qualitatively similar to the depression caused by these same reagents on the release of other leucocytic proteins; i.e., lysozyme and aldolase. The Rockefeller University Press 1964-05-01 /pmc/articles/PMC2137745/ /pubmed/14159044 Text en Copyright © 1964, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Berlin, Richard D. Wood, W. Barry STUDIES ON THE PATHOGENESIS OF FEVER : XII. ELECTROLYTIC FACTORS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUCOCYTES |
title | STUDIES ON THE PATHOGENESIS OF FEVER : XII. ELECTROLYTIC FACTORS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUCOCYTES |
title_full | STUDIES ON THE PATHOGENESIS OF FEVER : XII. ELECTROLYTIC FACTORS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUCOCYTES |
title_fullStr | STUDIES ON THE PATHOGENESIS OF FEVER : XII. ELECTROLYTIC FACTORS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUCOCYTES |
title_full_unstemmed | STUDIES ON THE PATHOGENESIS OF FEVER : XII. ELECTROLYTIC FACTORS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUCOCYTES |
title_short | STUDIES ON THE PATHOGENESIS OF FEVER : XII. ELECTROLYTIC FACTORS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUCOCYTES |
title_sort | studies on the pathogenesis of fever : xii. electrolytic factors influencing the release of endogenous pyrogen from polymorphonuclear leucocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137745/ https://www.ncbi.nlm.nih.gov/pubmed/14159044 |
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