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EFFECT OF ACTINOMYCIN D ON RNA SYNTHESIS AND ANTIBODY FORMATION IN THE ANAMNESTIC RESPONSE IN VITRO

Antibody synthesis in anamnestic lymphoid cells, measured by incorporation of leucine-C(14) into specific antibody, was inhibited at moderate concentrations of actinomycin D. This was accompanied by marked inhibition of synthesis of RNA as measured by incorporation of H(3)-cytidine monophosphate. Ho...

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Detalles Bibliográficos
Autores principales: Smiley, J. Donald, Heard, John G., Ziff, Morris
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1964
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137756/
https://www.ncbi.nlm.nih.gov/pubmed/19867234
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author Smiley, J. Donald
Heard, John G.
Ziff, Morris
author_facet Smiley, J. Donald
Heard, John G.
Ziff, Morris
author_sort Smiley, J. Donald
collection PubMed
description Antibody synthesis in anamnestic lymphoid cells, measured by incorporation of leucine-C(14) into specific antibody, was inhibited at moderate concentrations of actinomycin D. This was accompanied by marked inhibition of synthesis of RNA as measured by incorporation of H(3)-cytidine monophosphate. However, at low concentrations of actinomycin D, antibody synthesis was unaffected or even increased while RNA synthesis continued to be inhibited. The results obtained suggest that messenger RNA for antibody synthesis, either because it is relatively stable or present in excess, does not become a limiting factor until its synthesis is maximally inhibited. Puromycin, an inhibitor of amino acid coupling, abolished antibody synthesis in low concentration. 6-Mercaptopurine had no effect on the synthesis of antibody or RNA even at high concentration. The data obtained support the view that antibody synthesis follows pathways similar to those utilized for the formation of other types of proteins.
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spelling pubmed-21377562008-04-17 EFFECT OF ACTINOMYCIN D ON RNA SYNTHESIS AND ANTIBODY FORMATION IN THE ANAMNESTIC RESPONSE IN VITRO Smiley, J. Donald Heard, John G. Ziff, Morris J Exp Med Article Antibody synthesis in anamnestic lymphoid cells, measured by incorporation of leucine-C(14) into specific antibody, was inhibited at moderate concentrations of actinomycin D. This was accompanied by marked inhibition of synthesis of RNA as measured by incorporation of H(3)-cytidine monophosphate. However, at low concentrations of actinomycin D, antibody synthesis was unaffected or even increased while RNA synthesis continued to be inhibited. The results obtained suggest that messenger RNA for antibody synthesis, either because it is relatively stable or present in excess, does not become a limiting factor until its synthesis is maximally inhibited. Puromycin, an inhibitor of amino acid coupling, abolished antibody synthesis in low concentration. 6-Mercaptopurine had no effect on the synthesis of antibody or RNA even at high concentration. The data obtained support the view that antibody synthesis follows pathways similar to those utilized for the formation of other types of proteins. The Rockefeller University Press 1964-06-01 /pmc/articles/PMC2137756/ /pubmed/19867234 Text en Copyright © 1964, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Smiley, J. Donald
Heard, John G.
Ziff, Morris
EFFECT OF ACTINOMYCIN D ON RNA SYNTHESIS AND ANTIBODY FORMATION IN THE ANAMNESTIC RESPONSE IN VITRO
title EFFECT OF ACTINOMYCIN D ON RNA SYNTHESIS AND ANTIBODY FORMATION IN THE ANAMNESTIC RESPONSE IN VITRO
title_full EFFECT OF ACTINOMYCIN D ON RNA SYNTHESIS AND ANTIBODY FORMATION IN THE ANAMNESTIC RESPONSE IN VITRO
title_fullStr EFFECT OF ACTINOMYCIN D ON RNA SYNTHESIS AND ANTIBODY FORMATION IN THE ANAMNESTIC RESPONSE IN VITRO
title_full_unstemmed EFFECT OF ACTINOMYCIN D ON RNA SYNTHESIS AND ANTIBODY FORMATION IN THE ANAMNESTIC RESPONSE IN VITRO
title_short EFFECT OF ACTINOMYCIN D ON RNA SYNTHESIS AND ANTIBODY FORMATION IN THE ANAMNESTIC RESPONSE IN VITRO
title_sort effect of actinomycin d on rna synthesis and antibody formation in the anamnestic response in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137756/
https://www.ncbi.nlm.nih.gov/pubmed/19867234
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